Nebulizing conditions of pneumatic electrospray ionization significantly influence electrolyte effects on compound measurement

doi:10.1002/jms.2050

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	Nebulizing conditions of pneumatic electrospray ionization significantly influence electrolyte effects on compound measurement
	Niu, Wei 1; Zhu, Xiaohong 1,2; Yu, Ke 1; Li, Li 1; Sun, Yan 1; Li, Chuan1,2
刊名	JOURNAL OF MASS SPECTROMETRY
	2012-03
卷号	47 期号:3 页码:370-380
关键词	electrospray ionization electrolyte effects signal enhancement matrix effects upper limit of quantification ginkgo terpene lactone ginkgo flavonol
ISSN号	1076-5174
DOI	10.1002/jms.2050
文献子类	Article
英文摘要	Composition of mobile phase can greatly influence the success of electrospray ionization (ESI)-interfaced liquid chromatographymass spectrometry analysis. To investigate the relationship between formic-acid-based modification of mobile phase and ESI nebulizing conditions, an API 4000 ESI source and a TSQ Quantum one were compared under the same chromatographic conditions. Ginkgo terpene lactones and flavonols were measured in plasma, which involved using ascorbic acid to circumvent cross-interference between the analytes. ESI responses to using formic acid included changes in signal intensity, matrix effect, and upper limit of quantification. Significant disparities in the responses were observed between the two ESI sources, suggesting that the use of electrolyte modifier in liquid chromatography mobile phase and the pneumatic nebulization for ESI should be properly balanced to accomplish optimal ESI-based analysis. The distribution of unpaired ions toward the surface of the initial droplet was assumed to be an important step in the pneumatic ESI process. When using the electrolyte in mobile phase, a too fast droplet reduction by rapid-heating-assisted pneumatic nebulization could negatively decrease the time available for the unpaired ions to migrate from droplet interior to its surface. Ascorbic acid was identified as a major interfering substance for the bioanalytical assay; the interference mechanism might be associated with hindering the unpaired analyte ions from distributing toward the droplet surface rather than outcompeting the analyte ions for the limited excess charge on droplets surface. The current work extends the knowledge base of pneumatic ESI, which has implication for optimal use of the ESI-interfaced liquid chromatographymass spectrometry technique. Copyright (C) 2012 John Wiley & Sons, Ltd.
资助项目	National Science Fund of China for Distinguished Young Scholars[30925044] ; National Science and Technology Major Project of China "Key New Drug Creation and Manufacturing Program"[2009ZX09304-002] ; National Natural Science Foundation of China[3077277] ; National Basic Research Program of China[2012CB518403] ; Shanghai Science and Technology Major Projects[07DZ19703]
WOS关键词	CHROMATOGRAPHY-MASS SPECTROMETRY ; LASER-INDUCED FLUORESCENCE ; ELUENT COMPOSITION ; DRUG DISCOVERY ; PERFORMANCE ; PLASMA ; METABOLITES ; EFFICIENCY ; RETENTION ; DROPLETS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000301575200014
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278163]
专题	上海药物代谢研究中心科研与新药推进处
通讯作者	Li, Chuan
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Lab DMPK Res Herbal Med, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Grad Sch, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Niu, Wei,Zhu, Xiaohong,Yu, Ke,et al. Nebulizing conditions of pneumatic electrospray ionization significantly influence electrolyte effects on compound measurement[J]. JOURNAL OF MASS SPECTROMETRY,2012,47(3):370-380.
APA	Niu, Wei,Zhu, Xiaohong,Yu, Ke,Li, Li,Sun, Yan,&Li, Chuan.(2012).Nebulizing conditions of pneumatic electrospray ionization significantly influence electrolyte effects on compound measurement.JOURNAL OF MASS SPECTROMETRY,47(3),370-380.
MLA	Niu, Wei,et al."Nebulizing conditions of pneumatic electrospray ionization significantly influence electrolyte effects on compound measurement".JOURNAL OF MASS SPECTROMETRY 47.3(2012):370-380.