Characterization of a pectin from Lonicera japonica Thunb. and its inhibition effect on A beta(42) aggregation and promotion of neuritogenesis

doi:10.1016/j.ijbiomac.2017.08.154

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Characterization of a pectin from Lonicera japonica Thunb. and its inhibition effect on A beta(42) aggregation and promotion of neuritogenesis
	Liu, Qin 1,2,3,4; Fang, Jianping 3; Wang, Peipei1,3,4 ; Du, Zhenyun 1,3,4; Li, Yanling 1,3,4; Wang, Shunchun 2; Ding, Kan1,3,4
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2018-02
卷号	107 页码:112-120
关键词	Rhamnogalacturonan I (RG-I) pectic polysaccharide Lonicera japonica Thunb. A beta(42) aggregation Neuritogenesis Alzheimer's disease
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2017.08.154
文献子类	Article
英文摘要	Pectin is a class of complex polysaccharides and recognized for its potential bioactivities. In this study, we showed that a pectic polysaccharide, LFA03-a, was extracted from Lonicera japonica Thunb. flowers and purified with DEAE-celluiose and Sephacryl S-100HR. LFA03-a was composed of rhamnose, arabinose, galactose and galacturonic acid in the molar ratio of 18.1:25.3:36.8:19.5. Its structure was determined to possess a rhamnogalacturonan I (RG-I) backbone consisting of alpha-L-1,2-Rhap and alpha-D-1,4-GalAp disaccharide repeating unit, substituted at 0-4 of L-rhamnose. The side chain was involved with beta-D-1,4-Galp, beta-D-1,3-Galp, beta-D-1,3,6-Galp and branched alpha-L-1,5-Araf. Fluorescence spectroscopic analysis with thioflavine T (ThT) and atomic force microscopy (AFM) results showed that LFA03-a inhibited A beta(42) aggregation in a dose dependent manner and impeded A beta(42) oligomerization and fibril formation. In addition, LFA03-a mildly induced the differentiation of PC12 cells and promoted neuritogenesis.The results suggested that pectin LFA03-a might be a potential targeted therapeutic drug for Alzheimer's disease. (C) 2017 Published by Elsevier B.V.
资助项目	National Natural Science Foundation of China (NSFC)[31230022] ; Shanghai Outstanding Academic Leaders Program[16XD1404500] ; National Natural Science Foundation of China[31400690] ; Shanghai Science Research Program[13ZR1447500]
WOS关键词	NERVE GROWTH-FACTOR ; AMYLOID PEPTIDE NEUROTOXICITY ; CANCER CELL-PROLIFERATION ; BLOOD-BRAIN-BARRIER ; ALZHEIMERS-DISEASE ; STRUCTURAL-CHARACTERIZATION ; RHAMNOGALACTURONAN I ; NERIUM-INDICUM ; PHEOCHROMOCYTOMA CELLS ; LYCIUM-BARBARUM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000423892200013
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279932]
专题	药理学第三研究室
通讯作者	Wang, Shunchun; Ding, Kan
作者单位	1.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China; 2.Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, 1200 Cailun Rd, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Liu, Qin,Fang, Jianping,Wang, Peipei,et al. Characterization of a pectin from Lonicera japonica Thunb. and its inhibition effect on A beta(42) aggregation and promotion of neuritogenesis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,107:112-120.
APA	Liu, Qin.,Fang, Jianping.,Wang, Peipei.,Du, Zhenyun.,Li, Yanling.,...&Ding, Kan.(2018).Characterization of a pectin from Lonicera japonica Thunb. and its inhibition effect on A beta(42) aggregation and promotion of neuritogenesis.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,107,112-120.
MLA	Liu, Qin,et al."Characterization of a pectin from Lonicera japonica Thunb. and its inhibition effect on A beta(42) aggregation and promotion of neuritogenesis".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 107(2018):112-120.