Effects of Pro-Arg, a Novel Dipeptide Derived from Protamine Hydrolysate on H2O2-Induced Oxidative Stress in Human Diploid Fibroblasts

doi:10.1248/bpb.32.389

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Effects of Pro-Arg, a Novel Dipeptide Derived from Protamine Hydrolysate on H2O2-Induced Oxidative Stress in Human Diploid Fibroblasts
	Wang, Yonggang 1,2; Zhu, Fengrong 2; Chen, Jie 1; Han, Fusen 2; Wang, Heyao1
刊名	BIOLOGICAL & PHARMACEUTICAL BULLETIN
	2009-03
卷号	32 期号:3 页码:389-393
关键词	antioxidant dipeptide human diploid fibroblast reactive oxygen species
ISSN号	0918-6158
DOI	10.1248/bpb.32.389
文献子类	Article
英文摘要	Oxidative stress plays an important role in tissue damage associated with many different chronic degenerative diseases, such as cancer and cardiovascular disorders. In our previous study, it novel dipeptide Pro-Arg derived from protamine hydrolysates was found to possess significant antioxidative activity in vitro. In this study, we compared the antioxidant capabilities of purified and synthesized dipeptide (Pro-Arg) and found both of them had very strong hydroxyl-radical scavenging activity in vitro, even at a very low concentration. The protective effect of the dipeptide against oxidative stress was evaluated using H2O2-induced oxidative stress of human diploid fibroblasts MRC-5 cell model. Our results showed the dipeptide attenuated H2O2-induced cellular oxidative damage, and normalized S phase arrest of MRC-5 cells exposed to H2O2, Our findings demonstrate that Pro-Arg can protect against oxidative stress/damage and H2O2-induced human diploid fibroblasts cell death.
资助项目	National Supporting Project of the Eleventh Five-Year Plan[2006BAD27B06] ; National High Technology Research and Development Program of China[2007AA02Z301]
WOS关键词	L-ARGININE ; ANTIOXIDATIVE PEPTIDES ; SOYBEAN PROTEIN ; BEEF PATTIES ; AMINO-ACIDS ; IN-VITRO ; CARNOSINE ; INHIBITION ; PURIFICATION ; RESVERATROL
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	PHARMACEUTICAL SOC JAPAN
WOS记录号	WOS:000263777800014
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279298]
专题	药理学第三研究室
通讯作者	Wang, Heyao
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Global R&D Ctr Tianjin Tiens Grp, Tianjin 301700, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yonggang,Zhu, Fengrong,Chen, Jie,et al. Effects of Pro-Arg, a Novel Dipeptide Derived from Protamine Hydrolysate on H2O2-Induced Oxidative Stress in Human Diploid Fibroblasts[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2009,32(3):389-393.
APA	Wang, Yonggang,Zhu, Fengrong,Chen, Jie,Han, Fusen,&Wang, Heyao.(2009).Effects of Pro-Arg, a Novel Dipeptide Derived from Protamine Hydrolysate on H2O2-Induced Oxidative Stress in Human Diploid Fibroblasts.BIOLOGICAL & PHARMACEUTICAL BULLETIN,32(3),389-393.
MLA	Wang, Yonggang,et al."Effects of Pro-Arg, a Novel Dipeptide Derived from Protamine Hydrolysate on H2O2-Induced Oxidative Stress in Human Diploid Fibroblasts".BIOLOGICAL & PHARMACEUTICAL BULLETIN 32.3(2009):389-393.