A novel long non-coding RNA-ARA: Adriamycin Resistance Associated

doi:10.1016/j.bcp.2013.10.020

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第一研究室

	A novel long non-coding RNA-ARA: Adriamycin Resistance Associated
	Jiang, Min 1,4; Huang, Ou 4; Xie, Zuoquan5 ; Wu, Shuchao 5; Zhang, Xi 4; Shen, Aijun5 ; Liu, Hongchun5 ; Chen, Xiaosong 4; Wu, Jiayi 4; Lou, Ying 4
刊名	BIOCHEMICAL PHARMACOLOGY
	2014-01-15
卷号	87 期号:2 页码:254-283
关键词	Adriamycin Resistance Long non-coding RNA ARA: Adriamycin resistance associated Breast cancer Liver cancer
ISSN号	0006-2952
DOI	10.1016/j.bcp.2013.10.020
文献子类	Article
英文摘要	Long non-coding RNAs (IncRNAs) are emerging as an integral functional component of human genome and have been investigated as critical regulators in molecular biology of cancer. A recent study reported that IncRNA-UCA1 induced drug resistance in adriamycin chemotherapy. However, the contributions of IncRNAs to adriamycin resistance in cancers remain largely unknown. To address this issue, we performed a genome-wide IncRNA microarray analysis in adriamycin resistant MCF-7/ADR and parental MCF-7 cells, and revealed differential expression of IncRNAs in distinct category and chromosome distribution patterns. A specific differentially expressed IncRNA (Adriamycin Resistance Associated, termed ARA) was validated in MCF-7/ADR and HepG2/ADR cells. ARA is derived from an intron of PAK3 gene, predicted to contain several stable hairpins in secondary structure and has conservative sequences in primates. ARA expression is significantly associated with adriamycin sensitivity in a panel of breast and liver cancer cell lines and is markedly up-regulated in parental sensitive MCF-7 and HepG2 cell lines after receiving adriamycin treatment. The functions of ARA were assessed by silencing this IncRNA in vitro, and we found that ARA knockdown reduced the proliferation, induced cell death, G2/M arrest and migration defects. Furthermore, microarray transcriptomic analysis was carried out to comprehensively depict the ARA-regulated genes. We showed that ARA can modulate multiple signalling pathways, including MAPK signalling pathway, metabolism pathways, cell cycle and cell adhesion-related biological pathways, and regulate cellular processes, including transcriptional processes and protein binding function. Overall, our results indicate novel insights of adriamycin resistance in IncRNA level. (C) 2013 Elsevier Inc. All rights reserved.
资助项目	National Natured Science Foundation of China[81172520] ; National Natured Science Foundation of China[81202088] ; National Natured Science Foundation of China[81202549] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2012ZX09301001-007] ; Leading Academic Discipline Project of Shanghai Municipal Education Commission[J50208] ; Cancer Foundation of China[0901] ; Science and Technology Commission of Shanghai Municipality[09411961400] ; Shanghai Charity Cancer Research Center[00000000]
WOS关键词	BREAST-CANCER CELLS ; MULTIDRUG-RESISTANCE ; HEPATOCELLULAR-CARCINOMA ; DRUG-RESISTANCE ; GENE-EXPRESSION ; DOXORUBICIN ; APOPTOSIS ; EVOLUTION ; INVASION ; DISEASE
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000330332800005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277227]
专题	药理学第一研究室
通讯作者	Shen, Kunwei
作者单位	1.Soochow Univ, Affiliated Hosp 1, Dept Breast Surg, Suzhou 215006, Peoples R China; 2.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Dept Radiol, Shanghai 200025, Peoples R China 3.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Dept Endocrinol & Metab, Shanghai 200025, Peoples R China; 4.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Comprehens Breast Hlth Ctr, Shanghai 200025, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Jiang, Min,Huang, Ou,Xie, Zuoquan,et al. A novel long non-coding RNA-ARA: Adriamycin Resistance Associated[J]. BIOCHEMICAL PHARMACOLOGY,2014,87(2):254-283.
APA	Jiang, Min.,Huang, Ou.,Xie, Zuoquan.,Wu, Shuchao.,Zhang, Xi.,...&Shen, Kunwei.(2014).A novel long non-coding RNA-ARA: Adriamycin Resistance Associated.BIOCHEMICAL PHARMACOLOGY,87(2),254-283.
MLA	Jiang, Min,et al."A novel long non-coding RNA-ARA: Adriamycin Resistance Associated".BIOCHEMICAL PHARMACOLOGY 87.2(2014):254-283.