Pilot Study of (CuCl2)-Cu-64 for PET Imaging of Inflammation

doi:10.3390/molecules23020502

	Pilot Study of (CuCl2)-Cu-64 for PET Imaging of Inflammation
	Jiang, Lei 2,3,5; Song, Dongli 1; Chen, Hao 2,3; Zhang, Ao4 ; Wang, Huoqiang 5; Cheng, Zhen 2,3
刊名	MOLECULES
	2018-02
卷号	23 期号:2
关键词	(CuCl2)-Cu-64 copper transporter 1 (CTR1) PET inflammation
ISSN号	1420-3049
DOI	10.3390/molecules23020502
文献子类	Article
英文摘要	Copper(II) ion (Cu2+) is the essential element for numerous pathophysiological processes in vivo. Copper transporter 1 (CTR1) is mainly responsible for maintaining Cu2+ accumulation in cells, which has been found to be over-expressed in inflammatory tissues. Therefore, we explored the potential application of (CuCl2)-Cu-64 for PET imaging of inflammation through targeting CTR1. The animal models of H2O2 induced muscle inflammation and lipopolysaccaharide induced lung inflammation were successfully established, then imaged by small animal PET (PET/CT) post-injection of (CuCl2)-Cu-64, and PET images were quantitatively analyzed. H&E and immunohistochemical (IHC) staining and western blot experiments were performed for evaluating CTR1 levels in the inflammatory and control tissues. Both inflammatory muscle and lungs can be clearly imaged by PET. PET image quantitative analysis revealed that the inflammatory muscle and lungs showed significantly higher Cu-64 accumulation than the controls, respectively (p < 0.05). Furthermore, IHC staining and western blot analysis demonstrated that compared with the controls, CTR1 expression was increased in both the inflammatory muscle and lungs, which was consistent with the levels of Cu-64(2+) accumulation in these tissues. (CuCl2)-Cu-64 can be used as a novel, simple, and highly promising PET tracer for CTR1 targeted imaging of inflammation.
资助项目	International Cooperative Program of Chinese Academy of Sciences[GJHZ1622] ; National Science Foundation for Scholars of China[81571703] ; Outstanding Young Talents Program of Shanghai Municipal Commission of Health and Family Planning[2017YQ027] ; Shanghai Pujiang Program[2015PJD006]
WOS关键词	COPPER TRANSPORTER 1 ; HEPATOCELLULAR-CARCINOMA ; CU-64 UPTAKE ; MOUSE MODEL ; METABOLISM ; CANCER ; INJURY ; CELL ; HOMEOSTASIS ; EXPRESSION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000426436300282
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279920]
专题	药物化学研究室
通讯作者	Wang, Huoqiang; Cheng, Zhen
作者单位	1.Fudan Univ, Inst Clin Sci, Zhongshan Hosp, Shanghai 200032, Peoples R China; 2.Stanford Univ, Biox Program, Canary Ctr Stanford Canc Early Detect, Stanford, CA 94305 USA; 3.Stanford Univ, Dept Radiol, MIPS, Stanford, CA 94305 USA; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Pudong New Area, CAS Key Lab Receptor Res,SOMCL, 555 Zuchong Rd, Shanghai 201203, Peoples R China 5.Tongji Univ, Shanghai Pulm Hosp, Dept Nucl Med, Sch Med, Shanghai 200433, Peoples R China;
推荐引用方式 GB/T 7714	Jiang, Lei,Song, Dongli,Chen, Hao,et al. Pilot Study of (CuCl2)-Cu-64 for PET Imaging of Inflammation[J]. MOLECULES,2018,23(2).
APA	Jiang, Lei,Song, Dongli,Chen, Hao,Zhang, Ao,Wang, Huoqiang,&Cheng, Zhen.(2018).Pilot Study of (CuCl2)-Cu-64 for PET Imaging of Inflammation.MOLECULES,23(2).
MLA	Jiang, Lei,et al."Pilot Study of (CuCl2)-Cu-64 for PET Imaging of Inflammation".MOLECULES 23.2(2018).