Inducement of Cytokine Release by GFPBW2, a Novel Polysaccharide from Fruit Bodies of Grifola frondosa, through Dectin-1 in Macrophages

doi:10.1021/jf4029915

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Inducement of Cytokine Release by GFPBW2, a Novel Polysaccharide from Fruit Bodies of Grifola frondosa, through Dectin-1 in Macrophages
	Wang, Ying 1; Fang, Jianping 1; Ni, Xinyan 1; Li, Jie 1; Liu, Qin 1; Dong, Qun 1; Duan, Jinyou 2; Ding, Kan1
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2013-11-27
卷号	61 期号:47 页码:11400-11409
关键词	antitumor activity beta-glucan biological response modifier maitake
ISSN号	0021-8561
DOI	10.1021/jf4029915
文献子类	Article
英文摘要	Polysaccharides, especially beta-glucans isolated from various species of mushrooms, are considered as biological response modifiers (BRMs) to be widely used in the treatment of cancer, especially due to their immunostimulatory activity. We herein characterized the structure of a novel water-soluble homogeneous polysaccharide (GFPBW2) from the fruit bodies of mushroom Grifola frondosa and investigated its immunomodulatory activity in vitro. GFPBW2 was purified from the alkali-extracted fractions by stepwise elution with a molecular weight of 26.2 kDa. On the basis of infrared and NMR spectroscopy, methylation and monosaccharide composition analysis, partial acid hydrolysis, and Smith degradation, its structure was elucidated to possess a backbone consisting of beta-D-1,3- and beta-D-1,4-linked glucopyranosyl residues, with branches attached to O-6 of beta-D-1,3-linked glucopyranosyl residues. Functionally, it is an effective inducer of tumor necrosis factor-a (TNF-alpha) and interleukin-6 (IL-6) secretion in murine resident peritoneal macrophages. Using quartz crystal microbalance (QCM) analysis, we found that GFPBW2 could bind dendritic cell-associated C-type lectin-1 (Dectin-1) with an affinity constant (K-d) value of 1.08 X 10(-7) M, while it could activate Syk and enhance TNF-alpha production in RAW264.7 cells overexpressing wild type but not mutant Dectin-1. Furthermore, Syk, NF-kB signaling, and cytokine release in resident peritoneal macrophages induced by GFPBW2 could be significantly inhibited by a specific Dectin-1 blocking reagent, Laminarin. These data suggested that GFPBW2 might be a potential ligand of Dectin-1, and the potential of GFPBW2 to activate macrophage through triggering cytokine secretion might be attributed, at least in part, to the involvement of Dectin-1.
资助项目	New Drug Creation and Manufacturing Program[2012ZX09301001-003] ; National Science Fund for Distinguished Young Scholars[81125025] ; National Natural Science Foundation of China (NSFC)[31230022] ; National Natural Science Foundation of China (NSFC)[81171914]
WOS关键词	LUCIDUM FR. KARST ; BETA-D-GLUCAN ; NF-KAPPA-B ; GANODERMA-LUCIDUM ; SIGNALING PATHWAYS ; ANTITUMOR-ACTIVITY ; RECOGNITION ; INDUCTION ; RECEPTOR ; SPORES
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000327812500020
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277371]
专题	药理学第三研究室药物化学研究室
通讯作者	Duan, Jinyou
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China; 2.Northwest A&F Univ, Coll Sci, Yangling 712100, Shanxi, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ying,Fang, Jianping,Ni, Xinyan,et al. Inducement of Cytokine Release by GFPBW2, a Novel Polysaccharide from Fruit Bodies of Grifola frondosa, through Dectin-1 in Macrophages[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2013,61(47):11400-11409.
APA	Wang, Ying.,Fang, Jianping.,Ni, Xinyan.,Li, Jie.,Liu, Qin.,...&Ding, Kan.(2013).Inducement of Cytokine Release by GFPBW2, a Novel Polysaccharide from Fruit Bodies of Grifola frondosa, through Dectin-1 in Macrophages.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,61(47),11400-11409.
MLA	Wang, Ying,et al."Inducement of Cytokine Release by GFPBW2, a Novel Polysaccharide from Fruit Bodies of Grifola frondosa, through Dectin-1 in Macrophages".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 61.47(2013):11400-11409.