Metabolic sensor governing bacterial virulence in Staphylococcus aureus | |
Ding, Yue4; Liu, Xing5; Chen, Feifei4; Di, Hongxia4; Xu, Bin4; Zhou, Lu1; Deng, Xin2,6; Wu, Min3; Yang, Cai-Guang5; Lan, Lefu4 | |
刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
2014-11-18 | |
卷号 | 111期号:46页码:E4981-E4990 |
关键词 | Staphylococcus aureus metabolism iron acquisition virulence gene expression bacterial virulence |
ISSN号 | 0027-8424 |
DOI | 10.1073/pnas.1411077111 |
文献子类 | Article |
英文摘要 | An effective metabolism is essential to all living organisms, including the important human pathogen Staphylococcus aureus. To establish successful infection, S. aureus must scavenge nutrients and coordinate its metabolism for proliferation. Meanwhile, it also must produce an array of virulence factors to interfere with host defenses. However, the ways in which S. aureus ties its metabolic state to its virulence regulation remain largely unknown. Here we show that citrate, the first intermediate of the tricarboxylic acid (TCA) cycle, binds to and activates the catabolite control protein E (CcpE) of S. aureus. Using structural and site-directed mutagenesis studies, we demonstrate that two arginine residues (Arg145 and Arg256) within the putative inducer-binding cavity of CcpE are important for its allosteric activation by citrate. Microarray analysis reveals that CcpE tunes the expression of 126 genes that comprise about 4.7% of the S. aureus genome. Intriguingly, although CcpE is a major positive regulator of the TCA-cycle activity, its regulon consists predominantly of genes involved in the pathogenesis of S. aureus. Moreover, inactivation of CcpE results in increased staphyloxanthin production, improved ability to acquire iron, increased resistance to whole-blood-mediated killing, and enhanced bacterial virulence in a mouse model of systemic infection. This study reveals CcpE as an important metabolic sensor that allows S. aureus to sense and adjust its metabolic state and subsequently to coordinate the expression of virulence factors and bacterial virulence. |
资助项目 | National Natural Science Foundation of China[21472207] ; National Natural Science Foundation of China[31270126] ; National Natural Science Foundation of China[91313303] ; Shanghai Committee of Science and Technology[12JC1410200] ; Shanghai Committee of Science and Technology[12ZR1453200] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program"[2014ZX09507009-015] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program"[2013ZX09507-004] ; Hundred Talents Program of the Chinese Academy of Sciences[00000000] |
WOS关键词 | TRANSCRIPTIONAL REGULATORS ; CAPSULAR POLYSACCHARIDES ; PATHOGENIC BACTERIA ; CARBON METABOLISM ; BACILLUS-SUBTILIS ; STATIONARY-PHASE ; CYCLE ACTIVITY ; CELL-GROWTH ; IN-VITRO ; PROTEINS |
WOS研究方向 | Science & Technology - Other Topics |
语种 | 英语 |
出版者 | NATL ACAD SCIENCES |
WOS记录号 | WOS:000345153300013 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/276831] |
专题 | 药理学第三研究室 |
通讯作者 | Yang, Cai-Guang |
作者单位 | 1.Fudan Univ, Sch Pharm, Dept Med Chem, Shanghai 201203, Peoples R China; 2.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA; 3.Univ N Dakota, Sch Med & Hlth Sci, Dept Basic Sci, Grand Forks, ND 58203 USA 4.Chinese Acad Sci, Dept Mol Pharmacol, Shanghai 201203, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Shanghai 201203, Peoples R China; 6.Univ Chicago, Dept Chem, Chicago, IL 60637 USA; |
推荐引用方式 GB/T 7714 | Ding, Yue,Liu, Xing,Chen, Feifei,et al. Metabolic sensor governing bacterial virulence in Staphylococcus aureus[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2014,111(46):E4981-E4990. |
APA | Ding, Yue.,Liu, Xing.,Chen, Feifei.,Di, Hongxia.,Xu, Bin.,...&Lan, Lefu.(2014).Metabolic sensor governing bacterial virulence in Staphylococcus aureus.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,111(46),E4981-E4990. |
MLA | Ding, Yue,et al."Metabolic sensor governing bacterial virulence in Staphylococcus aureus".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 111.46(2014):E4981-E4990. |
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