(2S)-7,4 '-dihydroxy-8-prenylflavan stimulates adipogenesis and glucose uptake through p38MAPK pathway in 3T3-L1 cells

doi:10.1016/j.bbrc.2015.03.072

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	(2S)-7,4 '-dihydroxy-8-prenylflavan stimulates adipogenesis and glucose uptake through p38MAPK pathway in 3T3-L1 cells
	Ji, Jun 1,3; Zhu, Jingjie 3; Hu, Xiao 1; Wang, Ting 3; Zhang, Xiaodong 2; Hou, Ai-Jun 1; Wang, Heyao3
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2015-05-08
卷号	460 期号:3 页码:578-582
关键词	(2S)-7,4 '-dihydroxy-8-prenylflavan Adipogenesis Glucose uptake p38MAPK Type 2 diabetes
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2015.03.072
文献子类	Article
英文摘要	Adipose tissue plays a key role in the development of obesity and diabetes. Natural products are one of the main sources for discovering new lead compounds. In the present study, (2S)-7,4'-dihydroxy-8-prenylflavan (DHPF), a natural prenylated flavan isolated from Morus yunnanensis, was found to significantly promote adipogenesis and increase glucose uptake in 3T3-L1 cells. Real-time PCR results showed that DHPF increased the expression of glucose and lipid metabolism-related genes (C/EBP alpha, PPAR gamma, aP2, GLUT4 and adiponectin) and decreased the expression of inflammatory cytokine TNF-alpha. Western blotting further revealed that DHPF activated p38 MAPK at the initial stage of 3T3-L1 preadipocyte differentiation. DHPF-induced activation of p38, adipogenesis and glucose uptake were effectively blocked by SB203580, a specific p38 inhibitor. These results indicate that DHPF could stimulate adipogenesis and increase glucose uptake through the p38 MAPK pathway, and DHPF may be useful for the prevention and treatment of obesity-associated disorders such as type 2 diabetes (T2D). (C) 2015 Elsevier Inc. All rights reserved.
资助项目	National Natural Science Foundation of China[81473262] ; National Natural Science Foundation of China[81222045] ; National Natural Science Foundation of China[21072035] ; National Natural Science Foundation of China[21172044] ; Shu Guang project from Shanghai Municipal Education Commission and Shanghai Education Development Foundation[12SG02] ; National Science and Technology Major Project[2012ZX09301001-001]
WOS关键词	INSULIN-RESISTANCE ; ADIPOCYTE DIFFERENTIATION ; BROUSSONETIA-KAZINOKI ; PROMOTES ADIPOGENESIS ; DIABETES-MELLITUS ; ADIPOSE-TISSUE ; OBESITY ; PROTEIN ; THIAZOLIDINEDIONES ; INFLAMMATION
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000359885300015
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276534]
专题	药理学第三研究室
通讯作者	Wang, Heyao
作者单位	1.Fudan Univ, Sch Pharm, Dept Pharmacognosy, Shanghai 201203, Peoples R China; 2.Mayo Clin, Dept Biochem & Mol Biol, Scottsdale, AZ 85259 USA 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Ji, Jun,Zhu, Jingjie,Hu, Xiao,et al. (2S)-7,4 '-dihydroxy-8-prenylflavan stimulates adipogenesis and glucose uptake through p38MAPK pathway in 3T3-L1 cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,460(3):578-582.
APA	Ji, Jun.,Zhu, Jingjie.,Hu, Xiao.,Wang, Ting.,Zhang, Xiaodong.,...&Wang, Heyao.(2015).(2S)-7,4 '-dihydroxy-8-prenylflavan stimulates adipogenesis and glucose uptake through p38MAPK pathway in 3T3-L1 cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,460(3),578-582.
MLA	Ji, Jun,et al."(2S)-7,4 '-dihydroxy-8-prenylflavan stimulates adipogenesis and glucose uptake through p38MAPK pathway in 3T3-L1 cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 460.3(2015):578-582.