Effects of Phytoestrogens on Testosterone Production of Rat Leydig Cells

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	Effects of Phytoestrogens on Testosterone Production of Rat Leydig Cells
	ZHU Feng-rong 1; WANG Yong-gang 1; CHEN Jie 2; HU Yan-xue 1; HAN Fu-sen 1; WANG He-yao2
刊名	National Journal of Andrology
	2009
卷号	15 期号:3 页码:207-211
关键词	Leydig cell testosterone daidzein genistein P450 side-chain cleavage enzyme
ISSN号	1009-3591
其他题名	植物雌激素对体外培养的大鼠睾丸间质细胞分泌睾酮的作用
文献子类	Article
英文摘要	Objective: To investigate the effects of phytoestrogens (daidzein and genistein) on the testosterone production of rat Leydig cells and the possible mechanisms. Methods: Primary Ley dig cells were obtained from 3-month old male SD rats using discontinuous Percoll density gradient centrifugation. The effects of phytoestrogens at various concentrations were evaluated by ELISA, with hCG as the positive control. The mRNA expression of P450 side-chain cleavage enzyme (P450scc) was analyzed by semi-quantitative RT-PCR. Results: Genistein at 0.1 mumol/L obviously promoted the secretion of testosterone and upregulated the mRNA level of P450scc. At a higher concentration of 5 mumol/L, however, both daidzein and genistein significantly inhibited the testosterone production of Leydig cells (P〉0.05 ). Conclusion: Genistein can promote the testosterone production of Leydig cells at a low concentration (0.1 mumnol/L) , but both daidzein and genistein can inhibit it at a higher concentration (〉5 mumol/L).
资助项目	"十一五"国家科技支撑计划重点项目[00000000]
WOS研究方向	General & Internal Medicine (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:3543541
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/267972]
专题	药理学第三研究室
作者单位	1.Research Center, Tianjin Tiens Group, Tianjin 301700, China.; 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
推荐引用方式 GB/T 7714	ZHU Feng-rong,WANG Yong-gang,CHEN Jie,et al. Effects of Phytoestrogens on Testosterone Production of Rat Leydig Cells[J]. National Journal of Andrology,2009,15(3):207-211.
APA	ZHU Feng-rong,WANG Yong-gang,CHEN Jie,HU Yan-xue,HAN Fu-sen,&WANG He-yao.(2009).Effects of Phytoestrogens on Testosterone Production of Rat Leydig Cells.National Journal of Andrology,15(3),207-211.
MLA	ZHU Feng-rong,et al."Effects of Phytoestrogens on Testosterone Production of Rat Leydig Cells".National Journal of Andrology 15.3(2009):207-211.