Enhancing Membrane Protein Identification Using a Simplified Centrifugation and Detergent-Based Membrane Extraction Approach

doi:10.1021/acs.analchem.7b03710

	Enhancing Membrane Protein Identification Using a Simplified Centrifugation and Detergent-Based Membrane Extraction Approach
	Zhou, Yanting 1,4,5; Gao, Jing 1,5; Zhu, Hongwen 1,5; Xu, Jingjing 1,5; He, Han 1,5; Gu, Lei 4; Wang, Hui 4; Chen, Jie 1,5; Ma, Danjun 2,3; Zhou, Hu1,5
刊名	ANALYTICAL CHEMISTRY
	2018-02-20
卷号	90 期号:4 页码:2434-2439
ISSN号	0003-2700
DOI	10.1021/acs.analchem.7b03710
文献子类	Article
英文摘要	Membrane proteins may act as transporters, receptors, enzymes, and adhesion-anchors, accounting for nearly 70% of pharmaceutical drug targets. Difficulties in efficient enrichment, extraction, and solubilization still exist because of their relatively low abundance and poor solubility. A simplified membrane protein extraction approach with advantages of user-friendly sample processing procedures, good repeatability and significant effectiveness was developed in the current research for enhancing enrichment and identification of membrane proteins. This approach combining centrifugation and detergent along with LC-MS/MS successfully identified higher proportion of membrane proteins, integral proteins and transmembrane proteins in membrane fraction (76.6%, 48.1%, and 40.6%) than in total cell lysate (41.6%, 16.4%, and 13.5%), respectively. Moreover, our method tended to capture membrane proteins with high degree of hydrophobicity and number of transmembrane domains as 486 out of 2106 (23.0%) had GRAVY > 0 in membrane fraction, 488 out of 2106 (23.1%) had TMs >= 2. It also provided for improved identification of membrane proteins as more than 60.6% of the commonly identified membrane proteins in two cell samples were better identified in membrane fraction with higher sequence coverage. Data are available via ProteomeXchange with identifier PXD008456.
资助项目	National Key Research and Development Program from the Ministry of Science and Technology of China[2017YFC1700204] ; National Natural Science Foundation of China[21375138] ; National Natural Science Foundation of China[31570830] ; Strategic Priority Research Program of the Chinese Academy of Science, "Personalized Medicines-Molecular Signature-based Drug Discovery and Development"[XDA12030203] ; Innovation Project of Instrument and Equipment Function Development[YZ201542] ; Bureau of Goods, Chinese Academy of Sciences[00000000] ; Key Laboratory of Receptor Research, Chinese Academy of Sciences[00000000]
WOS关键词	MASS-SPECTROMETRY ; SAMPLE PREPARATION ; PROTEOMICS
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000426143100008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279895]
专题	分析化学研究室
通讯作者	Ma, Danjun; Zhou, Hu; Zheng, Jing
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 2.Dongguan Univ Technol, Coll Mech Engn, Dongguan 523808, Guangdong, Peoples R China; 3.Qingzi Biotechnol Shenzhen LLC, 4026 Shen Nan Middle Rd, Shenzhen 518039, Guangdong, Peoples R China 4.East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, Shanghai 200237, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Zhou, Yanting,Gao, Jing,Zhu, Hongwen,et al. Enhancing Membrane Protein Identification Using a Simplified Centrifugation and Detergent-Based Membrane Extraction Approach[J]. ANALYTICAL CHEMISTRY,2018,90(4):2434-2439.
APA	Zhou, Yanting.,Gao, Jing.,Zhu, Hongwen.,Xu, Jingjing.,He, Han.,...&Zheng, Jing.(2018).Enhancing Membrane Protein Identification Using a Simplified Centrifugation and Detergent-Based Membrane Extraction Approach.ANALYTICAL CHEMISTRY,90(4),2434-2439.
MLA	Zhou, Yanting,et al."Enhancing Membrane Protein Identification Using a Simplified Centrifugation and Detergent-Based Membrane Extraction Approach".ANALYTICAL CHEMISTRY 90.4(2018):2434-2439.