Cananga odorata essential oil reverses the anxiety induced by 1-(3-chlorophenyl) piperazine through regulating the MAPK pathway and serotonin system in mice

doi:10.1016/j.jep.2018.03.013

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第二研究室

	Cananga odorata essential oil reverses the anxiety induced by 1-(3-chlorophenyl) piperazine through regulating the MAPK pathway and serotonin system in mice
	Zhang, Nan 1; Zhang, Lei 2; Feng, Linyin2 ; Yao, Lei 1
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2018-06-12
卷号	219 页码:23-30
关键词	Cananga odorata 1-(3-Chlorophenyl) piperazine Anxiolytic effect MAPK 5-HT
ISSN号	0378-8741
DOI	10.1016/j.jep.2018.03.013
文献子类	Article
英文摘要	Ethnopharmacological relevance: Cananga odorata essential oil, known as ylang-ylang essential oil (YYO), was commonly used in the aromatherapy for relaxation and mood adjusting use. In our previous study, YYO played anxiolytic effects on the mice in several behavioral tests that based on the instinctive responses to novel environments. Aim of the study: To investigate the effects and mechanisms of YYO reversing the anxiety induced by 5-HT2C receptor agonist 1-(3-chlorophenyl) piperazine (m-CPP). Materials and methods: m-CPP was administrated to the male ICR mice to develop an anxiety model. The anxiolytic effect of YYO (0.1%, 1% and 10%, v/v) was evaluated in the elevated plus maze (EPM) test after odor exposure. Western blot was used to detect the phosphorylation levels of extracellular signal-regulated kinase 1/2 (ERK1/2) and cAMP response element-binding protein (CREB) and the expression of c-Fos in the prefrontal cortex (PFC) and hippocampus after the EPM test. Serotonin and its metabolite change in the brain were detected by liquid chromatogram with an electrochemical detector. The effect of YYO on the plasma corticosterone level was evaluated using enzyme-linked immunosorbent assay (ELISA) after the odor exposure. Results: The behavior analysis showed that m-CPP (2 mg/kg and 4 mg/kg) could induce anxiety behaviors in the mice while diazepam (2 mg/kg) reversed the anxiety behavior induced by m-CPP. YYO dose-dependently increased the time and number of entries in the open arms (p < 0.05) compared to the Tween 80 group. YYO reduced the phosphorylation levels of ERK1/2 (p < 0.05) in both PFC and hippocampus. Down-regulations of phosphor-CREB (p < 0.05) and c-Fos (p < 0.05) were only observed in the hippocampus. YYO also affected the brain serotonin metabolism and reduced the blood plasma corticosterone level of the m-CPP treated mice. Conclusion: YYO odor exposure could reverse the anxiety behaviors generated by m-CPP. The anxiolytic effect of YYO was associated with the ERK1/2/CREB pathway in the hippocampus and relevant to the serotonin system.
资助项目	Shanghai Jiao Tong University[00000000]
WOS关键词	5-HT2C RECEPTOR ANTAGONIST ; MCPP-INDUCED ANXIETY ; RAT-BRAIN ; YLANG-YLANG ; PLUS-MAZE ; C-FOS ; ACTIVATION ; STRESS ; CHLOROPHENYLPIPERAZINE ; COMPLEMENTARY
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000434901400003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279709]
专题	药理学第二研究室
通讯作者	Feng, Linyin; Yao, Lei
作者单位	1.Shanghai Jiao Tong Univ, Sch Agr & Biol, 800 Dong Chuan Rd, Shanghai, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Nan,Zhang, Lei,Feng, Linyin,et al. Cananga odorata essential oil reverses the anxiety induced by 1-(3-chlorophenyl) piperazine through regulating the MAPK pathway and serotonin system in mice[J]. JOURNAL OF ETHNOPHARMACOLOGY,2018,219:23-30.
APA	Zhang, Nan,Zhang, Lei,Feng, Linyin,&Yao, Lei.(2018).Cananga odorata essential oil reverses the anxiety induced by 1-(3-chlorophenyl) piperazine through regulating the MAPK pathway and serotonin system in mice.JOURNAL OF ETHNOPHARMACOLOGY,219,23-30.
MLA	Zhang, Nan,et al."Cananga odorata essential oil reverses the anxiety induced by 1-(3-chlorophenyl) piperazine through regulating the MAPK pathway and serotonin system in mice".JOURNAL OF ETHNOPHARMACOLOGY 219(2018):23-30.