Cynomorium songaricum Extracts Functionally Modulate Transporters of gamma-Aminobutyric Acid and Monoamine

doi:10.1007/s11064-009-0118-2

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 天然药物化学研究室

	Cynomorium songaricum Extracts Functionally Modulate Transporters of gamma-Aminobutyric Acid and Monoamine
	Zhao, Gang 1,2; Wang, Jie 1; Qin, Guo-Wei 3; Guo, Li-He 1,2
刊名	NEUROCHEMICAL RESEARCH
	2010-04
卷号	35 期号:4 页码:666-676
关键词	Cynomorium songaricum Rupr gamma-Aminobutyric acid Dopamine Norepinephrine Serotonin Transporter modulator
ISSN号	0364-3190
DOI	10.1007/s11064-009-0118-2
文献子类	Article
英文摘要	Cynomorium songaricum Rupr. (SY) is a central nervous system-oriented herb material that has actions of anti-dementia, anti-epilepsy, and anti-stress. It is unclear whether SY would be biologically active in functionally regulating neurotransmitter transporters. Here, we assessed these potential actions using Chinese hamster ovary cells expressing gamma-aminobutyric acid (GABA) transporter (GAT-1), dopamine transporter (DAT), norepinephrine transporter (NET), or serotonin transporter (SERT) (i.e. G1, D8, N1, or S6 cells, respectively). It was shown that SY extracts, such as SYw, SYa, SYp, SYc, SYe, and SYb (SY water, ethanol, petroleum ether, chloroform, ethyl acetate, and n-butyl alcohol extract, respectively) increased dopamine/norepinephrine (DA/NE) uptake by corresponding D8/N1 cells and decreased gamma-aminobutyric acid/serotoin (GABA/5HT) uptake by corresponding G1/S6 cells; wherein, the potency or efficacy of SYc for up-regulating DA/NE uptake and that of SYb for inhibiting GABA/5HT uptake were relatively stronger. Additionally, GABA/5HT-uptake inhibition by SY extracts were also seen in cortical synaptosomes, and DA/NE-uptake enhancement by SYc was dependent on the activity of DAT and NET. Thus, SY extracts especially SYc and SYb are novel neurotransmitter-transporter modulators functioning as DAT/NET activators and/or GAT-1/SERT inhibitors.
资助项目	Shanghai Government[05DZ19339] ; Chinese Academy of Science[00000000]
WOS关键词	DOPAMINE TRANSPORTER ; SEROTONIN TRANSPORTER ; NORADRENALINE TRANSPORTER ; GABA TRANSPORTER ; CONSTITUENTS ; INHIBITION ; MECHANISMS ; ADDICTION ; STEMS ; L.
WOS研究方向	Biochemistry & Molecular Biology ; Neurosciences & Neurology
语种	英语
出版者	SPRINGER/PLENUM PUBLISHERS
WOS记录号	WOS:000275820500020
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278937]
专题	天然药物化学研究室
通讯作者	Guo, Li-He
作者单位	1.Cell Star Biotechnol Co Ltd, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Gang,Wang, Jie,Qin, Guo-Wei,et al. Cynomorium songaricum Extracts Functionally Modulate Transporters of gamma-Aminobutyric Acid and Monoamine[J]. NEUROCHEMICAL RESEARCH,2010,35(4):666-676.
APA	Zhao, Gang,Wang, Jie,Qin, Guo-Wei,&Guo, Li-He.(2010).Cynomorium songaricum Extracts Functionally Modulate Transporters of gamma-Aminobutyric Acid and Monoamine.NEUROCHEMICAL RESEARCH,35(4),666-676.
MLA	Zhao, Gang,et al."Cynomorium songaricum Extracts Functionally Modulate Transporters of gamma-Aminobutyric Acid and Monoamine".NEUROCHEMICAL RESEARCH 35.4(2010):666-676.