Characterization of major flavonoids, triterpenoid, dipeptide and their metabolites in rat urine after oral administration of Radix Astragali decoction

doi:10.1134/S1061934813080145

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物质量控制与固体化学研究中心

	Characterization of major flavonoids, triterpenoid, dipeptide and their metabolites in rat urine after oral administration of Radix Astragali decoction
	Zhang, Z. Y.1,3; Wu, B.1; Tang, Y. H.1; Li, Z. X.1; Sun, Z. L.1; Chen, L. Y.1; Ji, Y. B.2,3; Ma, C. H.1; Huang, C. G.1
刊名	JOURNAL OF ANALYTICAL CHEMISTRY
	2013-08
卷号	68 期号:8 页码:716-721
关键词	Astragalus membranaceus metabolites urine HPLC-MS/MS
ISSN号	1061-9348
DOI	10.1134/S1061934813080145
文献子类	Article
英文摘要	To characterize of the constituents in rat urine after oral administration of Radix Astragali decoction, a HPLC-DAD-MS/MS technique had been developed. Urine collected from 0 to 24 h, after administration, was purified using a C18 solid-phase extraction cartridge, and then detected by an on-line MS/MS detector. By comparing the retention times and MS/MS data with those obtained from authentic compounds and the published data, a total of 11 compounds including six flavonoids, one dipeptide, one triterpenoid and three of their metabolites in urine were identified. Compounds daidzein, genistein, quercetin, L-asparamide-D-phenylalanine, 4,2',4'-trihydroxychalcone, Astragaloside-IV, daidzein sulfate, and kaempferol sulfate were for the first time detected by HPLC-MS/MS technique in urine. The present research results success-fully narrowed the range of effective constituents to be found in Astragalus membranaceus and would be useful for the following action mechanism research of this traditional Chinese medicine in treating various diseases.
资助项目	National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program, China"[2009ZX09301-001] ; Science and Technology Commission of Shanghai Municipality[08DZ1971402]
WOS关键词	IONIZATION-MASS-SPECTROMETRY ; LC-MS ; IDENTIFICATION ; ISOFLAVONES ; SAPONINS ; MS/MS ; SERUM
WOS研究方向	Chemistry
语种	英语
出版者	MAIK NAUKA/INTERPERIODICA/SPRINGER
WOS记录号	WOS:000321769800008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277527]
专题	药物质量控制与固体化学研究中心
通讯作者	Zhang, Z. Y.
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Tradit Chinese Med Modernizat Ctr, Shanghai 201203, Peoples R China; 2.Natl Minist Educ, Nat Antitumor Drug Engn Res Ctr, Harbin 150076, Peoples R China 3.Harbin Univ Commerce, Life Sci & Environm Sci Res Ctr, Harbin 150076, Peoples R China;
推荐引用方式 GB/T 7714	Zhang, Z. Y.,Wu, B.,Tang, Y. H.,et al. Characterization of major flavonoids, triterpenoid, dipeptide and their metabolites in rat urine after oral administration of Radix Astragali decoction[J]. JOURNAL OF ANALYTICAL CHEMISTRY,2013,68(8):716-721.
APA	Zhang, Z. Y..,Wu, B..,Tang, Y. H..,Li, Z. X..,Sun, Z. L..,...&Huang, C. G..(2013).Characterization of major flavonoids, triterpenoid, dipeptide and their metabolites in rat urine after oral administration of Radix Astragali decoction.JOURNAL OF ANALYTICAL CHEMISTRY,68(8),716-721.
MLA	Zhang, Z. Y.,et al."Characterization of major flavonoids, triterpenoid, dipeptide and their metabolites in rat urine after oral administration of Radix Astragali decoction".JOURNAL OF ANALYTICAL CHEMISTRY 68.8(2013):716-721.