The Mechanism by Which Luteolin Disrupts the Cytoplasmic Membrane of Methicillin-Resistant Staphylococcus aureus

doi:10.1021/acs.jpcb.7b05766

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	The Mechanism by Which Luteolin Disrupts the Cytoplasmic Membrane of Methicillin-Resistant Staphylococcus aureus
	Zhang, Tao 1; Qiu, Yunguang 2,3; Luo, Qichao 2,3; Zhao, Lifen 2; Yan, Xin 2,4; Ding, Qiaoce 2; Jiang, Hualiang1,2,3 ; Yang, Huaiyu 2,3
刊名	JOURNAL OF PHYSICAL CHEMISTRY B
	2018-02-01
卷号	122 期号:4 页码:1427-1438
ISSN号	1520-6106
DOI	10.1021/acs.jpcb.7b05766
文献子类	Article
英文摘要	Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most versatile :human pathogens. Luteolin (LUT) has anti-MRSA activity by disrupting the MRSA cytoplasmic membrane. However, the mechanism by which luteolin disrupts the Membrane remains unclear. Here, we performed differential:scanning calorimetry (DSC) and all atomic molecular dynamics (AA-MD) simulations to investigate the interactions and effects of LUT on model membranes composed of phosphatidyleholine (PC) and phosphatidylglycerol,(PG). We detected the transition thermodynamic parameters of dipalmitoylphosphatidylcholine (DPPC) liposomes) dipalrnitoylphosphatidylglycerol (DPPG) liposomes, and liposomes composed of both DPPC and DPPG at different LUT concentrations and showed that LUT molecules were located between polar heads and the hydrophobic region, of DPPC/DPPG. In the MD trajectories, LUT molecules ranging from 5 to 50 had different effects on the membranes thickness, fluidity and ordered property of lipids, and lateral pressure of lipid bilayers composed of dioleoylphosphatidylcholine (DOPC) and dioleoylphosphatidylglycerol (DOPG). Also, most LUT molecules were distributed in the region between the phosphorus atoms and C9 atoms of DOPC and DOPG. On the basis of the combination of these results, we conclude that the distinct effects of LUT on lipid bilayers composed of PCs and PGs may elucidate the mechanism by which LUT disrupts the cytoplasmic membrane of MRSA.
资助项目	National Natural Science Foundation of China[21422208] ; E-Institutes of Shanghai Municipal Education Commission[E09013] ; Institutes for Drug Discovery and Development, Chinese Academy of Sciences[00000000] ; Special Program for Applied Research on Super Computation of the NSFC-Guangdong Joint Fund[U1501501]
WOS关键词	MOLECULAR-DYNAMICS SIMULATIONS ; FLAVONOID-BIOMEMBRANE INTERACTIONS ; LIPID-MEMBRANES ; PHOSPHOLIPID-MEMBRANES ; ESCHERICHIA-COLI ; PHASE-TRANSITION ; NATURAL-PRODUCTS ; BILAYERS ; MODEL ; CHOLESTEROL
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000424731000009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279928]
专题	药物发现与设计中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Jiang, Hualiang; Yang, Huaiyu
作者单位	1.East China Univ Sci & Technol, Sch Pharm, 130 Meilong Rd, Shanghai 200237, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Drug Discovery & Design Ctr, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China; 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China; 4.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Huaxiazhong Rd, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Tao,Qiu, Yunguang,Luo, Qichao,et al. The Mechanism by Which Luteolin Disrupts the Cytoplasmic Membrane of Methicillin-Resistant Staphylococcus aureus[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2018,122(4):1427-1438.
APA	Zhang, Tao.,Qiu, Yunguang.,Luo, Qichao.,Zhao, Lifen.,Yan, Xin.,...&Yang, Huaiyu.(2018).The Mechanism by Which Luteolin Disrupts the Cytoplasmic Membrane of Methicillin-Resistant Staphylococcus aureus.JOURNAL OF PHYSICAL CHEMISTRY B,122(4),1427-1438.
MLA	Zhang, Tao,et al."The Mechanism by Which Luteolin Disrupts the Cytoplasmic Membrane of Methicillin-Resistant Staphylococcus aureus".JOURNAL OF PHYSICAL CHEMISTRY B 122.4(2018):1427-1438.