AMPK regulates anaphase central spindle length by phosphorylation of KIF4A

doi:10.1093/jmcb/mjx029

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	AMPK regulates anaphase central spindle length by phosphorylation of KIF4A
	Li, Qian-Ru 2; Yan, Xiu-Min 3; Guo, Lin 1; Li, Jia2 ; Zang, Yi2
刊名	JOURNAL OF MOLECULAR CELL BIOLOGY
	2018-02
卷号	10 期号:1 页码:2-17
关键词	AMPK phosphoregulation KIF4A mitosis central spindle length
ISSN号	1674-2788
DOI	10.1093/jmcb/mjx029
文献子类	Article
英文摘要	AMP-activated protein kinase (AMPK) is an energy sensor that couples the cellular energy state with basic biological processes. AMPK is thought to be linked with cell division although the underlying mechanisms remain largely unknown. Here, we show that AMPK functionally participates throughout cell division and that AMPK catalytic subunits, especially alpha 2, are sequentially associated with separate mitotic apparatus. Using quantitative phosphoproteomics analysis, we found that the strong direct substrate KIF4A is phosphorylated by AMPK at Ser801. Further analysis revealed that AMPK and Aurora B competitively phosphoregulates KIF4A during mitotic phase due to overlapping recognition motifs, resulting in the elaborate phosphoregulation for KIF4A-dependent central spindle length control. Given the intrinsic energy-sensing function of AMPK, our study links the KIF4A-dependent control of central spindle length with cellular glucose stress.
资助项目	National Natural Science Foundation of China[81673489] ; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences[SIMM1705ZZ-02] ; Institutes for Drug Discovery and Development, Chinese Academy of Sciences[CASIMM0120161001] ; Science and Technology Commission of Shanghai Municipality[16430724100] ; Science and Technology Commission of Shanghai Municipality[14431902800]
WOS关键词	ACTIVATED PROTEIN-KINASE ; CHROMOSOME CONDENSATION ; MIDZONE FORMATION ; ENERGY SENSOR ; MITOTIC EXIT ; MICROTUBULE GENERATION ; CHROMOKINESIN KIF4A ; UPSTREAM KINASE ; HUMAN-CELLS ; HISTONE H3
WOS研究方向	Cell Biology
语种	英语
CSCD记录号	CSCD:6201803
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000428634600002
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279915]
专题	国家新药筛选中心中科院受体结构与功能重点实验室新药研究国家重点实验室药物安全性评价中心
通讯作者	Guo, Lin; Li, Jia; Zang, Yi
作者单位	1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Hubei, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci,State Key Lab Cel, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Li, Qian-Ru,Yan, Xiu-Min,Guo, Lin,et al. AMPK regulates anaphase central spindle length by phosphorylation of KIF4A[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2018,10(1):2-17.
APA	Li, Qian-Ru,Yan, Xiu-Min,Guo, Lin,Li, Jia,&Zang, Yi.(2018).AMPK regulates anaphase central spindle length by phosphorylation of KIF4A.JOURNAL OF MOLECULAR CELL BIOLOGY,10(1),2-17.
MLA	Li, Qian-Ru,et al."AMPK regulates anaphase central spindle length by phosphorylation of KIF4A".JOURNAL OF MOLECULAR CELL BIOLOGY 10.1(2018):2-17.