iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation

doi:10.1038/s41419-018-0561-6

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物发现与设计中心

	iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation
	Gao, Kun 1,3; Zhang, Yuanyuan 2; Shi, Qing 1; Zhang, Jianong 1; Zhang, Liang 1; Sun, Huiru 1; Jiao, Dongyue 1; Zhao, Xiayin 1; Tao, Hongru 2; Wei, Youheng 1
刊名	CELL DEATH & DISEASE
	2018-05-09
卷号	9
ISSN号	2041-4889
DOI	10.1038/s41419-018-0561-6
文献子类	Article
英文摘要	Cytokinesis is the last step of cell division and is concluded by the abscission of the intercellular bridge that connects two daughter cells The tight regulation of cytokinesis completion is essential because cytokinesis failure is associated with various human diseases Here, we report that iASPP, a member of the apoptosis stimulating proteins of p53 (ASPP) family, is required for proper cell division iASPP depletion results in abnormal midbody structure and failed cytokinesis We used protein affinity purification methods to identify the functional partners of iASPP We found that iASPP associates with centrosomal protein of 55 kDa (CEP55), an important cytokinetic abscission regulator Mechanically, iASPP acts as a PP1 targeting subunit to facilitate the interaction between PP1 and CEP55 and to remove PLK1 mediated Ser436 phosphorylation in CEP55 during late mitosis The latter step is critical for the timely recruitment of CEP55 to the midbody The present observations revealed a previously unrecognized function of iASPP in cytokinesis This function, in turn, likely contributes to the roles of iASPP in tumor development and genetic diseases.
资助项目	National Natural Science Foundation of China[81672558] ; National Natural Science Foundation of China[81201533] ; National Natural Science Foundation of China[81572768] ; National Natural Science Foundation of China[31400753] ; National Key Research and Development Plan of China-Precision Medicine Project[2016YFC0902202] ; Shanghai municipal medical and health discipline construction projects[2017ZZ02015]
WOS关键词	ESCRT MACHINERY ; MITOTIC EXIT ; COILED-COIL ; CELL-CYCLE ; MIDBODY ; PROTEIN ; FAMILY ; CARDIOMYOPATHY ; RECRUITMENT ; MECHANISMS
WOS研究方向	Cell Biology
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000431788600008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279763]
专题	药物发现与设计中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Gao, Kun; Zhang, Pingzhao; Wang, Chenji
作者单位	1.Fudan Univ, Sch Life Sci, Inst Biomed Sci, State Key Lab Genet Engn, Shanghai, Peoples R China; 2.Chinese Acad Sci, Drug Discovery & Design Ctr, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 3.Tongji Univ, Shanghai Matern & Infant Hosp 1, Clin & Translat Res Ctr, Sch Med, Shanghai, Peoples R China;
推荐引用方式 GB/T 7714	Gao, Kun,Zhang, Yuanyuan,Shi, Qing,et al. iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation[J]. CELL DEATH & DISEASE,2018,9.
APA	Gao, Kun.,Zhang, Yuanyuan.,Shi, Qing.,Zhang, Jianong.,Zhang, Liang.,...&Wang, Chenji.(2018).iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.CELL DEATH & DISEASE,9.
MLA	Gao, Kun,et al."iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation".CELL DEATH & DISEASE 9(2018).