Acid-Promoted D-A-D Type Far-Red Fluorescent Probe with High Photostability for Lysosomal Nitric Oxide Imaging

doi:10.1021/acs.analchem.8b00612

	Acid-Promoted D-A-D Type Far-Red Fluorescent Probe with High Photostability for Lysosomal Nitric Oxide Imaging
	Wang, Fengyang 2; Yu, Shujuan 3; Xu, Zhiai 2; Li, Lingling 2; Dang, Yijing 2; Xu, Xiaowei 4; Luo, Yi 4; Cheng, Zhen 1,5; Yu, Haijun6 ; Zhang, Wen 2
刊名	ANALYTICAL CHEMISTRY
	2018-07-03
卷号	90 期号:13 页码:7953-7962
ISSN号	0003-2700
DOI	10.1021/acs.analchem.8b00612
文献子类	Article
英文摘要	To accurately monitor the variations of lysosomal nitric oxide (NO) under physiological condition remains a great challenge for understanding the biological function of NO. Herein, we developed a new chemotype probe, namely, MBTD, for acid-promoted and far-red fluorescence imaging of lysosomal NO in vitro and ex vivo. MBTD was rationally designed by incorporating o-phenyl-enediamino (OPD) moiety into the donor-acceptor-donor (D-A-D) type fluorophore based on a dual intramolecular charge transfer (ICT) mechanism. Compared to previously reported OPD-based NO probes, MBTD displays several distinct advantages including large stroke shift, huge on-off ratio with minimal autofluorescence, and high NO specificity. Particularly, MBTD exhibits an acid-promoted response to NO with high acid tolerance, which greatly improves the spatial resolution to lysosomal NO by excluding the background noise from other nonacidic organelles. Furthermore, MBTD displayed much longer-lived and more stable fluorescence emission in comparison with the commercialized NO probe. MBTD was employed for ratiometric examination of the exogenous or endogenous NO of macrophages. More importantly, MBTD was able to detect the variation of lysosomal NO level in an acute liver injury mouse model ex vivo, implying the potential of MBTD for real-time monitoring the therapeutic efficacy of drug candidates for the treatment of acute liver injury. MBTD as a novel type of NO probe might open a new avenue for precisely sensing lysosomal NO-related pathological and therapeutic process.
资助项目	National Natural Science Foundation of China[21675055] ; National Natural Science Foundation of China[81402790] ; National Natural Science Foundation of China[21775046] ; National Natural Science Foundation of China[21635003] ; International Cooperative Program of the Chinese Academy of Sciences[GJHZ1622] ; Key Program of the Frontier Science of the Chinese Academy of Sciences[160621]
WOS关键词	LIVING CELLS ; SELECTIVE DETECTION ; HYDROGEN-SULFIDE ; QUANTUM DOTS ; LIVE CELLS ; AUTOPHAGY ; TISSUES ; DISEASE ; HEALTH ; DYE
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000438008600025
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279668]
专题	药物化学研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Xu, Zhiai; Ding, Chunyong
作者单位	1.Stanford Univ, Dept Radiol, Stanford, CA 94305 USA; 2.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Synthet Organ & Med Chem Lab, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 4.Dalian Univ Technol, Sch Chem Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China; 5.Stanford Univ, BioX Program, MIPS, Stanford, CA 94305 USA; 6.Chinese Acad Sci, SIMM, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wang, Fengyang,Yu, Shujuan,Xu, Zhiai,et al. Acid-Promoted D-A-D Type Far-Red Fluorescent Probe with High Photostability for Lysosomal Nitric Oxide Imaging[J]. ANALYTICAL CHEMISTRY,2018,90(13):7953-7962.
APA	Wang, Fengyang.,Yu, Shujuan.,Xu, Zhiai.,Li, Lingling.,Dang, Yijing.,...&Ding, Chunyong.(2018).Acid-Promoted D-A-D Type Far-Red Fluorescent Probe with High Photostability for Lysosomal Nitric Oxide Imaging.ANALYTICAL CHEMISTRY,90(13),7953-7962.
MLA	Wang, Fengyang,et al."Acid-Promoted D-A-D Type Far-Red Fluorescent Probe with High Photostability for Lysosomal Nitric Oxide Imaging".ANALYTICAL CHEMISTRY 90.13(2018):7953-7962.