Improved Expression of His(6)-Tagged Strictosidine Synthase cDNA for Chemo-Enzymatic Alkaloid Diversification

doi:10.1002/cbdv.201000052

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 新药研究国家重点实验室

	Improved Expression of His(6)-Tagged Strictosidine Synthase cDNA for Chemo-Enzymatic Alkaloid Diversification
	Yang, Liuqing 1,2; Zou, Hongbin 1,2; Zhu, Huajian 1; Ruppert, Martin 2; Gong, Jingxu 3; Stoeckigt, Joachim 1,2
刊名	CHEMISTRY & BIODIVERSITY
	2010
卷号	7 期号:4 页码:860-870
ISSN号	1612-1872
DOI	10.1002/cbdv.201000052
文献子类	Article
英文摘要	Strictosichne synthase (sTRI) catalyzes the stereoselective formation of 3 alpha(S)-strictosidine from tryptamine and secologanin. Strictosidine is the key intermediate in the biosynthesis of 2,000 plant monoterpenoid indole alkaloids, and it is a key precursor of enzyme-mediated synthesis of alkaloids. An improved expression system is described which leads to optimized His(6)-STR1 synthesis in Escherichia coli. Optimal production of STRI was achieved by determining the impact of co-expression of chaperones pG-Tf2 and pG-LJES. The amount and activity of sTRI was doubled in the presence of chaperone pG-Tf2 alone. His(6)-STRI immobilized on Ni-NTA can be used for enzymatic synthesis of strictosidines on a preparative scale. With the newly co-expressed His(6)-STRI. novel 3 alpha(5)-12-azastrictosidine was obtained by enzymatic catalysis of 7-azatryptamine and secologanin. The results obtained are of significant importance for application to chemo-enzymatic approaches leading to diversification of alkaloids with novel improved structures.
WOS关键词	INDOLE ALKALOIDS ; ESCHERICHIA-COLI ; KEY INTERMEDIATE ; NATURAL-PRODUCTS ; ENZYME ; BIOSYNTHESIS ; RAUVOLFIA
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000277250200005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279015]
专题	新药研究国家重点实验室中科院受体结构与功能重点实验室
通讯作者	Zou, Hongbin
作者单位	1.Zhejiang Univ, Inst Mat Med, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China; 2.Johannes Gutenberg Univ Mainz, Dept Pharmaceut Biol, Inst Pharm & Biochem, D-55128 Mainz, Germany; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yang, Liuqing,Zou, Hongbin,Zhu, Huajian,et al. Improved Expression of His(6)-Tagged Strictosidine Synthase cDNA for Chemo-Enzymatic Alkaloid Diversification[J]. CHEMISTRY & BIODIVERSITY,2010,7(4):860-870.
APA	Yang, Liuqing,Zou, Hongbin,Zhu, Huajian,Ruppert, Martin,Gong, Jingxu,&Stoeckigt, Joachim.(2010).Improved Expression of His(6)-Tagged Strictosidine Synthase cDNA for Chemo-Enzymatic Alkaloid Diversification.CHEMISTRY & BIODIVERSITY,7(4),860-870.
MLA	Yang, Liuqing,et al."Improved Expression of His(6)-Tagged Strictosidine Synthase cDNA for Chemo-Enzymatic Alkaloid Diversification".CHEMISTRY & BIODIVERSITY 7.4(2010):860-870.