Preparative isolation of triterpenoids from Ganoderma lucidum by counter-current chromatography combined with pH-zone-refining

doi:10.1016/j.foodchem.2011.07.122

	Preparative isolation of triterpenoids from Ganoderma lucidum by counter-current chromatography combined with pH-zone-refining
	Cheng, Chun-Ru; Li, Yi-Feng; Xu, Ping-Ping; Feng, Rui-Hong; Yang, Min; Guan, Shu-Hong; Guo, De-An
刊名	FOOD CHEMISTRY
	2012-02-15
卷号	130 期号:4 页码:1010-1016
关键词	Ganoderic acid Ganoderma lucidum pH-zone-refining CCC Stepwise CCC Triterpenoid
ISSN号	0308-8146
DOI	10.1016/j.foodchem.2011.07.122
文献子类	Article
英文摘要	Ganoderma lucidum is a famous fungus. The triterpenoids are the main bioactive components and exhibit various pharmacological activities. However, the scarcity of the pure triterpenoid has greatly restrained the modern research of G. lucidum. The present paper first describes a convenient method to separate the main triterpenoids from G. lucidum using counter-current chromatography (CCC) technique. Ganoderic acid C6 (38 mg), E (76 mg), F (32 mg) were successfully separated from the crude extract in 1 day with the HPLC purity of above 90% using stepwise CCC. Ganoderic acid G (36 mg), A (64 mg), B (25 mg), D (28 mg) and ganoderenic acid D (11 mg) were separated in 2 days with the HPLC purity of above 90% using a combination of stepwise CCC and pH-zone-refining CCC. The method presented in the paper is much quicker and easier than the previous methods. (C) 2011 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China (NNSFC)[30701077] ; ministry of Sciences and Technology of China[2006BAI08B03-03] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09308-005] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09311-001] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09502-020] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX 09304-002] ; Chinese Academy of Sciences[KSCX2-YW-R-166] ; Ministry of Science and Technology of the People's Republic of China[00000000]
WOS关键词	FR KARST ; STEPWISE ELUTION ; CONSTITUENTS ; ACID ; PURIFICATION ; SEPARATION
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000296124600033
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278182]
专题	成果转移转化处上海中药现代化研究中心
通讯作者	Guan, Shu-Hong
作者单位	Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med,Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Chun-Ru,Li, Yi-Feng,Xu, Ping-Ping,et al. Preparative isolation of triterpenoids from Ganoderma lucidum by counter-current chromatography combined with pH-zone-refining[J]. FOOD CHEMISTRY,2012,130(4):1010-1016.
APA	Cheng, Chun-Ru.,Li, Yi-Feng.,Xu, Ping-Ping.,Feng, Rui-Hong.,Yang, Min.,...&Guo, De-An.(2012).Preparative isolation of triterpenoids from Ganoderma lucidum by counter-current chromatography combined with pH-zone-refining.FOOD CHEMISTRY,130(4),1010-1016.
MLA	Cheng, Chun-Ru,et al."Preparative isolation of triterpenoids from Ganoderma lucidum by counter-current chromatography combined with pH-zone-refining".FOOD CHEMISTRY 130.4(2012):1010-1016.