Application of Histidine Kinase (YycG) in Screening Inhibitor of Streptococcus pneumoniae

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	Application of Histidine Kinase (YycG) in Screening Inhibitor of Streptococcus pneumoniae
	LI Nan 3; WANG Fei 2; NIU Si-qiang 3; WANG Hong 3; LI You-qiang 3; YIN Nan-lin 3; ZHANG Xue-mei 3; ZHU Wei-liang1 ; YIN Yin-bing 3; XUN Wen-chun 3
刊名	Journal of Chinese Biotechnology
	2009
卷号	29 期号:3 页码:25-29
关键词	Histidine kinase Purified protein Enzymatic activity Screening in vitro
ISSN号	1671-8135
其他题名	组氨酸激酶(YycG)在筛选肺炎链球菌抑制剂中的应用
文献子类	Article
英文摘要	To obtain a protein fragment of Streptococcus pneumoniae histidine kinase YycG with in vitro ATP -dependent kinase activity for screening novel bacterial inhibitors, the genomic DNA encoding the kinase domain of YycG was expressed in E. coli BL21 (DE3 ). The product was identified by SDS - PAGE and Western blot assays, purified by a His -tagged Ni affinity column, and determined for kinase activity. It was about 35 kDa, reaching a purity of 95% , and has dose - dependent kinase activity of hydrolyzing ATP in vitro. Based on the inhibitory action on the catalyzing function of the purified kinase protein, several effective inhibitors were screened out from 105 candidate compounds available, one of which was selected out and verified overt bactericidal effects in vitro. Collectively, a fragment of histidine kinase YycG expressed in vitro was successful used to screen kinase inhibitors that may be important micro - molecule compound for developing antibacterial drugs or disinfectant.
资助项目	国家自然科学基金[00000000] ; 重庆市自然科学基金[00000000]
WOS研究方向	Biochemistry & Molecular Biology (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:3608756
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/267985]
专题	药物发现与设计中心
作者单位	1.Faculty of Laboratory Medicine,Shanghai Institute of Material Medical, Graduate School of Chinese Academy of Sciences, Shanghai 201203, China. 2.Shanghai Institute of Material Medical, Graduate School of Chinese Academy of Sciences, Shanghai 201203, China.; 3.Faculty of Laboratory Medicine,Chongqing Medical University, Chongqing 400016.;
推荐引用方式 GB/T 7714	LI Nan,WANG Fei,NIU Si-qiang,et al. Application of Histidine Kinase (YycG) in Screening Inhibitor of Streptococcus pneumoniae[J]. Journal of Chinese Biotechnology,2009,29(3):25-29.
APA	LI Nan.,WANG Fei.,NIU Si-qiang.,WANG Hong.,LI You-qiang.,...&XUN Wen-chun.(2009).Application of Histidine Kinase (YycG) in Screening Inhibitor of Streptococcus pneumoniae.Journal of Chinese Biotechnology,29(3),25-29.
MLA	LI Nan,et al."Application of Histidine Kinase (YycG) in Screening Inhibitor of Streptococcus pneumoniae".Journal of Chinese Biotechnology 29.3(2009):25-29.