Actin Polymerization-Dependent Increase in Synaptic Arc/Arg3.1 Expression in the Amygdala Is Crucial for the Expression of Aversive Memory Associated with Drug Withdrawal

doi:10.1523/JNEUROSCI.0871-12.2012

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第二研究室

	Actin Polymerization-Dependent Increase in Synaptic Arc/Arg3.1 Expression in the Amygdala Is Crucial for the Expression of Aversive Memory Associated with Drug Withdrawal
	Liu, Yao 2; Zhou, Qi-Xin 1; Hou, Yuan-Yuan 2; Lu, Bin 2; Yu, Chuan 2; Chen, Jie 2; Ling, Qing-Lan 2; Cao, Jun 1; Chi, Zhi-Qiang2 ; Xu, Lin 1
刊名	JOURNAL OF NEUROSCIENCE
	2012-08-29
卷号	32 期号:35 页码:12005-12017
ISSN号	0270-6474
DOI	10.1523/JNEUROSCI.0871-12.2012
文献子类	Article
英文摘要	Aversive memories associated with drug withdrawal may contribute to persistent drug seeking. Molecular mechanisms that are critical for aversive memory formation have yet to be elucidated. Recently, we showed in a rat conditioned place aversion (CPA) model that synaptic actin polymerization in the amygdala were required for aversive memory information. Here, we demonstrated that actin polymerization within the amygdala triggered transportation of activity-regulated cytoskeletal-associated protein (Arc/Arg3.1) into amygdalar synapses. Increased synaptic Arc/Arg3.1 expression contributed to aversive memory formation by regulating synaptic AMPA receptor (AMPAR) endocytosis, as in vivo knockdown of amygdalar Arc/Arg3.1 with Arc/Arg3.1-shRNA prevented both AMPAR endocytosis and CPA formation. We also demonstrated that conditioned morphine withdrawal led to induction of LTD in the amygdala through AMPAR endocytosis. We further demonstrated that Arc/Arg3.1-regulated AMPAR endocytosis was GluR2 dependent, as intra-amygdala injection of Tat-GluR2(3Y), a GluR2-derived peptide that has been shown to specifically block regulated, but not constitutive, AMPAR endocytosis, prevented AMPAR endocytosis, LTD induction, and aversive memory formation. Therefore, this study extends previous studies on the role of actin polymerization in synaptic plasticity and memory formation by revealing the critical molecular events involved in aversive memory formation as well as LTD induction, and by showing that Arc/Arg3.1 is a crucial mediator for actin polymerization functions, and, thus, underscores the unknown details of how actin polymerization mediates synaptic plasticity and memory.
资助项目	National Basic Research Program[00000000] ; Ministry of Science and Technology of China[2009CB522005] ; Ministry of Science and Technology of China[2009CB522006] ; Ministry of Science and Technology of China[2009CB941302] ; Foundation of National Natural Science of China[81130087] ; Foundation of National Natural Science of China[81001424] ; Foundation of National Natural Science of China[31100775] ; Foundation of National Natural Science of China[U1032605] ; Foundation of National Natural Science of China[U1132602] ; Chinese Academy of Sciences[KSCX2-YW-R-253]
WOS关键词	AMPA RECEPTOR TRAFFICKING ; LONG-TERM POTENTIATION ; BEHAVIORAL SENSITIZATION ; NUCLEUS-ACCUMBENS ; HIPPOCAMPAL-NEURONS ; LATERAL AMYGDALA ; DENDRITIC SPINES ; PLACE AVERSION ; MESSENGER-RNA ; IN-VIVO
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	SOC NEUROSCIENCE
WOS记录号	WOS:000308213900009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277983]
专题	药理学第二研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Liu, Jing-Gen
作者单位	1.Chinese Acad Sci, Kunming Inst Zool, Lab Learning & Memory, Kunming 65022, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Liu, Yao,Zhou, Qi-Xin,Hou, Yuan-Yuan,et al. Actin Polymerization-Dependent Increase in Synaptic Arc/Arg3.1 Expression in the Amygdala Is Crucial for the Expression of Aversive Memory Associated with Drug Withdrawal[J]. JOURNAL OF NEUROSCIENCE,2012,32(35):12005-12017.
APA	Liu, Yao.,Zhou, Qi-Xin.,Hou, Yuan-Yuan.,Lu, Bin.,Yu, Chuan.,...&Liu, Jing-Gen.(2012).Actin Polymerization-Dependent Increase in Synaptic Arc/Arg3.1 Expression in the Amygdala Is Crucial for the Expression of Aversive Memory Associated with Drug Withdrawal.JOURNAL OF NEUROSCIENCE,32(35),12005-12017.
MLA	Liu, Yao,et al."Actin Polymerization-Dependent Increase in Synaptic Arc/Arg3.1 Expression in the Amygdala Is Crucial for the Expression of Aversive Memory Associated with Drug Withdrawal".JOURNAL OF NEUROSCIENCE 32.35(2012):12005-12017.