BMS309403 Stimulates Glucose Uptake in Myotubes through Activation of AMP-Activated Protein Kinase

doi:10.1371/journal.pone.0044570

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	BMS309403 Stimulates Glucose Uptake in Myotubes through Activation of AMP-Activated Protein Kinase
	Lin, Wanhua 3; Huang, Xiaoli 3; Zhang, Lina 1; Chen, Dongmei 1; Wang, Dongye 3; Peng, Qilong 4; Xu, Lei 1; Li, Jingya1 ; Liu, Xiujie 4; Li, Kuai 3
刊名	PLOS ONE
	2012-08-31
卷号	7 期号:8
ISSN号	1932-6203
DOI	10.1371/journal.pone.0044570
文献子类	Article
英文摘要	BMS309403 is a biphenyl azole inhibitor against fatty acid binding protein 4 (FABP4) and regarded as a lead compound for effective treatment of obesity related cardio-metabolic diseases. Here we discovered an off-target activity of BMS309403 in that it stimulates glucose uptake in C2C12 myotubes in a temporal and dose dependent manner via activation of AMP-activated protein kinase (AMPK) signaling pathway but independent of FABPs. Further analysis indicated that BMS309403 activates AMPK through increasing the ratio of intracellular AMP:ATP while decreasing mitochondrial membrane potential. These findings provide mechanistic insights on the action of BMS309403.
资助项目	National Basic Research Program of China (973 Program)[2011CB504004] ; National Basic Research Program of China (973 Program)[2010CB945500] ; strategic program of stem cell (Chinese Academy of Sciences)[XDA01020303] ; National Science Foundation of China[30970637] ; National Science Foundation of China[30810099] ; National Science Foundation of China[90813033] ; National Science Foundation of China[30811120429]
WOS关键词	ACID-BINDING PROTEIN ; SKELETAL-MUSCLE ; CASCADE ; GLUT4 ; ALPHA ; CELLS
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000308221300099
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277981]
专题	国家新药筛选中心中科院受体结构与功能重点实验室新药研究国家重点实验室药理学第一研究室药物安全性评价中心
通讯作者	Lin, Wanhua
作者单位	1.Chinese Acad Sci, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai Inst Mat Med, Guangzhou, Guangdong, Peoples R China; 2.Univ Florida, Dept Mol Genet & Microbiol, Gainesville, FL USA 3.Chinese Acad Sci, Key Lab Regenerat Biol, Guangzhou Inst Biomed & Hlth, Guangzhou, Guangdong, Peoples R China; 4.Chinese Acad Sci, Inst Biol Chem, Guangzhou Inst Biomed & Hlth, Guangzhou, Guangdong, Peoples R China;
推荐引用方式 GB/T 7714	Lin, Wanhua,Huang, Xiaoli,Zhang, Lina,et al. BMS309403 Stimulates Glucose Uptake in Myotubes through Activation of AMP-Activated Protein Kinase[J]. PLOS ONE,2012,7(8).
APA	Lin, Wanhua.,Huang, Xiaoli.,Zhang, Lina.,Chen, Dongmei.,Wang, Dongye.,...&Wu, Donghai.(2012).BMS309403 Stimulates Glucose Uptake in Myotubes through Activation of AMP-Activated Protein Kinase.PLOS ONE,7(8).
MLA	Lin, Wanhua,et al."BMS309403 Stimulates Glucose Uptake in Myotubes through Activation of AMP-Activated Protein Kinase".PLOS ONE 7.8(2012).