Senkyunolide I attenuates oxygen-glucose deprivation/reoxygenation-induced inflammation in microglial cells

doi:10.1016/j.brainres.2016.08.012

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	Senkyunolide I attenuates oxygen-glucose deprivation/reoxygenation-induced inflammation in microglial cells
	Hu, Yang-ye 1,2; Wang, Yuan 1; Liang, Shuang 1; Yu, Xue-li 3; Zhang, Lei 3; Feng, Lin-yin3 ; Feng, Yi 1
刊名	BRAIN RESEARCH
	2016-10-15
卷号	1649 页码:123-131
关键词	Senkyunolide I Microglial cells Oxygen-glucose deprivation/reoxygenation TLR4/NF-kappa B pathway HSF-1/Hsp70
ISSN号	0006-8993
DOI	10.1016/j.brainres.2016.08.012
文献子类	Article
英文摘要	Over-activated microglia during stroke has been documented to aggravate brain damage. Our previous studies showed that senkyunolide I (SEI) exerted anti-inflammatory effects against endotoxin insult in vitro and ameliorative effects on cerebral ischemia/reperfusion (I/R) injury in vivo. Using oxygen-glucose deprivation/reoxygenation (OGD/R) to mimic stroke, we here investigated the anti-inflammatory effect of SEI on microglial cells and explored the underlying mechanisms. OGD for 3 h followed by reoxygenation for 12 h significantly enhanced the release of pro-inflammatory cytokines and expressions of inflammation-related enzymes in BV-2 cells, which was inhibited by pretreatment with SEI. To elucidate the mechanisms, we studied its effect on upstream signaling pathways. It was found that SEI suppressed the activation of NE-kappa B pathway induced by OGD/R and the MAPK pathway was shown not to be involved. Furthermore, SEI significantly down-regulated TLR4/MyD88 pathway with specifically improving inducible Hsp70 level through increasing HSF-1/DNA binding activity, and these regulations responsive to SEI were attenuated by transfecting Hsp70 siRNA and HSF-1 decoy ODNs. Additionally, SEI exerted similar influence on Hsp70/TLR4/NF-kappa B pathway in rat primary microglial cells. The results suggested that SEI had a potent effect against stroke-induced neuroinflammation through suppressing the TLR4/NE-kappa B pathway by up-regulating Hsp70 dependent on HSF-1. (C) 2016 Elsevier B.V. All rights reserved.
资助项目	Chinese Ministry of Education[211060] ; Sci-Tech Innovation Item of Shanghai Education Committee[12ZZ124] ; Research Fund for the Doctoral Program of Higher Education of China[20123107120009]
WOS关键词	NECROSIS-FACTOR-ALPHA ; INTERCELLULAR-ADHESION MOLECULE-1 ; FOCAL CEREBRAL-ISCHEMIA ; HEAT-SHOCK PROTEINS ; FACTOR-KAPPA-B ; INNATE IMMUNITY ; ISCHEMIA/REPERFUSION INJURY ; SIGNAL-TRANSDUCTION ; BRAIN-INJURY ; ACTIVATION
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000386408900013
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275854]
专题	药理学第二研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Feng, Lin-yin; Feng, Yi
作者单位	1.Shanghai Univ Tradit Chinese Med, Engn Res Ctr Modern Preparat TCM, Minist Educ, Shanghai, Peoples R China; 2.Shanghai Univ Tradit Chinese Med, TCM Syndrome & Syst Biol Res Ctr, Shanghai, Peoples R China; 3.Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yang-ye,Wang, Yuan,Liang, Shuang,et al. Senkyunolide I attenuates oxygen-glucose deprivation/reoxygenation-induced inflammation in microglial cells[J]. BRAIN RESEARCH,2016,1649:123-131.
APA	Hu, Yang-ye.,Wang, Yuan.,Liang, Shuang.,Yu, Xue-li.,Zhang, Lei.,...&Feng, Yi.(2016).Senkyunolide I attenuates oxygen-glucose deprivation/reoxygenation-induced inflammation in microglial cells.BRAIN RESEARCH,1649,123-131.
MLA	Hu, Yang-ye,et al."Senkyunolide I attenuates oxygen-glucose deprivation/reoxygenation-induced inflammation in microglial cells".BRAIN RESEARCH 1649(2016):123-131.