Isolation of chlorogenic acids and their derivatives from Stemona japonica by preparative HPLC and evaluation of their anti-AIV (H5N1) activity in vitro

doi:10.1002/pca.974

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 天然药物化学研究室

	Isolation of chlorogenic acids and their derivatives from Stemona japonica by preparative HPLC and evaluation of their anti-AIV (H5N1) activity in vitro
	Ge, Fan ; Ke, Changqiang; Tang, Wei; Yang, Xinzhou ; Tang, Chunping; Qin, Guowei ; Xu, Rensheng ; Li, Tianxian ; Chen, Xinwen ; Zuo, Jianping
刊名	PHYTOCHEMICAL ANALYSIS
	2007-05
卷号	18 期号:3 页码:213-218
关键词	chlorogenic acids Stemona japonica preparative HPLC Anti-AIV (H5NI) activity
ISSN号	0958-0344
DOI	10.1002/pca.974
文献子类	Article
英文摘要	Two chlorogenic acids and five chlorogenic acid derivatives were simultaneously separated and purified from Stemona japonica by preparative high-performance liquid chromatography. Five of the collected compounds were over 95% pure while the other two compounds were over 90% pure. Their structures were elucidated as 3-O-feruloylquinic acid (1), 4-O-feruloylquinic acid (2), methyl 3-O-feruloylquinate (3), methyl 5-O-caffeyolquinate (4), methyl 4-O-feruloylquinate (5), ethyl 3-O-feruloylquinate (6) and the new compound ethyl 4-O-feruloylquinate (7) by UV, NMR and ESI-MS. All compounds were obtained from Stemona species for the first time, however compounds 6 and 7 are believed to be artefacts from the ethanol extraction. The anti-AIV (H5N1) activities were evaluated by Neutral Red uptake assay. Compounds 3 and 4 exerted moderate inhibitory effect against AIV (H5N1) in vitro. Copyright (c) 2007 John Wiley & Sons, Ltd.
WOS关键词	QUINIC ACID ; SEPARATION ; CHROMATOGRAPHY ; CONSTITUENTS
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry
语种	英语
出版者	JOHN WILEY & SONS LTD
WOS记录号	WOS:000246163300006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273278]
专题	天然药物化学研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Ye, Yang
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Ge, Fan,Ke, Changqiang,Tang, Wei,et al. Isolation of chlorogenic acids and their derivatives from Stemona japonica by preparative HPLC and evaluation of their anti-AIV (H5N1) activity in vitro[J]. PHYTOCHEMICAL ANALYSIS,2007,18(3):213-218.
APA	Ge, Fan.,Ke, Changqiang.,Tang, Wei.,Yang, Xinzhou.,Tang, Chunping.,...&Ye, Yang.(2007).Isolation of chlorogenic acids and their derivatives from Stemona japonica by preparative HPLC and evaluation of their anti-AIV (H5N1) activity in vitro.PHYTOCHEMICAL ANALYSIS,18(3),213-218.
MLA	Ge, Fan,et al."Isolation of chlorogenic acids and their derivatives from Stemona japonica by preparative HPLC and evaluation of their anti-AIV (H5N1) activity in vitro".PHYTOCHEMICAL ANALYSIS 18.3(2007):213-218.