Phosphoglycerate mutase 1 promotes cancer cell migration independent of its metabolic activity

doi:10.1038/onc.2016.446

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第一研究室

	Phosphoglycerate mutase 1 promotes cancer cell migration independent of its metabolic activity
	Zhang, D.1; Jin, N.1; Sun, W.1; Li, X.1; Liu, B.2; Xie, Z.1; Qu, J.1; Xu, J.1; Yang, X.1; Su, Y.1
刊名	ONCOGENE
	2017-05-18
卷号	36 期号:20 页码:2900-2909
ISSN号	0950-9232
DOI	10.1038/onc.2016.446
文献子类	Article
英文摘要	Phosphoglycerate mutase 1 (PGAM1) is a glycolytic enzyme that coordinates glycolysis and biosynthesis to promote cancer growth via its metabolic activity. Here, we report the discovery of a non-metabolic function of PGAM1 in promoting cancer metastasis. A proteomic study identified a-smooth muscle actin (ACTA2) as a PGAM1-associated protein. PGAM1 modulated actin filaments assembly, cell motility and cancer cell migration via directly interacting with ACTA2, which was independent of its metabolic activity. The enzymatically inactive H186R mutant retained its association with ACTA2, whereas 201-210 amino acids deleted PGAM1 mutant lost the interaction with ACTA2 regardless of intact metabolic activity. Importantly, PGAM1 knockdown decreased metastatic potential of breast cancer cells in vivo and PGAM1 and ACTA2 were jointly associated with the prognosis of breast cancer patients. Together, this study provided the first evidence revealing a non-metabolic function of PGAM1 in promoting cell migration, and gained new insights into the role of PGAM1 in cancer progression.
资助项目	China International Science and Technology Cooperation Program[2015DFM30040] ; National Science and Technology Major Project[2015ZX09101009] ; National Natural Science Foundation of China[81573464] ; National Natural Science Foundation of China[81321092] ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1406402]
WOS关键词	PYRUVATE-KINASE M2 ; LUNG ADENOCARCINOMA ; ACTIN CYTOSKELETON ; GENE-TRANSCRIPTION ; CREATINE-KINASE ; CARCINOMA ; GLYCOLYSIS ; WARBURG ; TARGET ; GROWTH
WOS研究方向	Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000401697800012
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272660]
专题	药理学第一研究室中科院受体结构与功能重点实验室新药研究国家重点实验室药物化学研究室
通讯作者	Huang, M.; Geng, M.
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Room 2431,555 Zuchongzhi Rd,Room 1320, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Chem Prote Ctr, State Key Lab Drug Res, Shanghai, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Pharmaceut Anal, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, D.,Jin, N.,Sun, W.,et al. Phosphoglycerate mutase 1 promotes cancer cell migration independent of its metabolic activity[J]. ONCOGENE,2017,36(20):2900-2909.
APA	Zhang, D..,Jin, N..,Sun, W..,Li, X..,Liu, B..,...&Geng, M..(2017).Phosphoglycerate mutase 1 promotes cancer cell migration independent of its metabolic activity.ONCOGENE,36(20),2900-2909.
MLA	Zhang, D.,et al."Phosphoglycerate mutase 1 promotes cancer cell migration independent of its metabolic activity".ONCOGENE 36.20(2017):2900-2909.