EXPRESSION OF A CDNA FOR A NEURONAL CALCIUM-CHANNEL ALPHA-1 SUBUNIT ENHANCES SECRETION FROM ADRENAL CHROMAFFIN CELLS

	EXPRESSION OF A CDNA FOR A NEURONAL CALCIUM-CHANNEL ALPHA-1 SUBUNIT ENHANCES SECRETION FROM ADRENAL CHROMAFFIN CELLS
	MA, WJ ; HOLZ, RW ; UHLER, MD
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	1992-11-15
卷号	267 期号:32 页码:22728-22732
ISSN号	0021-9258
文献子类	Article
英文摘要	A synthetic oligonucleotide was used to isolate mouse brain cDNA clones coding for a brain isoform of the alpha1 subunit of the voltage-sensitive Ca2+ channel. Twenty-six independent cDNA clones were isolated and sequenced. All the cDNA clones reported here showed high homology to the rat brain class C cDNA sequence (Snutch, T. P., Tomlinson, W. J., Leonard, J. P., and Gilbert, M. M. (1991) Neuron 7, 45-57). Comparison of the individual mouse brain class C (mbC) cDNA sequences indicated the presence of four regions within the alpha1 subunit coding sequence where alternative splicing can take place in mouse brain and raise the possibility that combinatorial arrangement of these splice variants could give rise to a heterogenous class of mbC transcripts. Northern blot analysis demonstrated that mbC mRNA sequences could be detected in highest abundance in mouse heart, at lower levels in mouse brain and spinal cord, and not at all in liver or skeletal muscle. An expression vector for one isoform of the mbC alpha1 subunit cDNA was constructed using the human cytomegalovirus promoter to direct expression, and this expression vector was used in a novel transfection assay of primary cultures of bovine adrenal chromaffin cells. Transfection of the mbC alpha1 subunit expression vector increased the secretion of human growth hormone derived from a cotransfected human growth hormone expression vector after stimulation with elevated K+ or the dihydropyridine agonist, Bay K8644. These experiments suggest that this isoform of the mbC alpha1 subunit is functional in the transfected chromaffin cells and that the number of Ca2+ channels is a limiting component in the secretion from chromaffin cells in culture.
资助项目	NIDDK NIH HHS[DK27959]
WOS关键词	DEPENDENT PROTEIN-KINASE ; HUMAN GROWTH-HORMONE ; FUNCTIONAL EXPRESSION ; SKELETAL-MUSCLE ; GENE-EXPRESSION ; CA2+ CHANNELS ; IDENTIFICATION ; EXOCYTOSIS ; ACTIVATION ; RECEPTOR
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:A1992JY16300008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275145]
专题	中国科学院上海药物研究所
作者单位	1.UNIV MICHIGAN,DEPT PHARMACOL,ANN ARBOR,MI 48109 2.CHINESE ACAD SCI,SHANGHAI INST MATERIA MED,SHANGHAI,PEOPLES R CHINA 3.UNIV MICHIGAN,MENTAL HLTH RES INST,205 ZINA PITCHER PL,ANN ARBOR,MI 48109 4.UNIV MICHIGAN,DEPT BIOL CHEM,ANN ARBOR,MI 48109
推荐引用方式 GB/T 7714	MA, WJ,HOLZ, RW,UHLER, MD. EXPRESSION OF A CDNA FOR A NEURONAL CALCIUM-CHANNEL ALPHA-1 SUBUNIT ENHANCES SECRETION FROM ADRENAL CHROMAFFIN CELLS[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,1992,267(32):22728-22732.
APA	MA, WJ,HOLZ, RW,&UHLER, MD.(1992).EXPRESSION OF A CDNA FOR A NEURONAL CALCIUM-CHANNEL ALPHA-1 SUBUNIT ENHANCES SECRETION FROM ADRENAL CHROMAFFIN CELLS.JOURNAL OF BIOLOGICAL CHEMISTRY,267(32),22728-22732.
MLA	MA, WJ,et al."EXPRESSION OF A CDNA FOR A NEURONAL CALCIUM-CHANNEL ALPHA-1 SUBUNIT ENHANCES SECRETION FROM ADRENAL CHROMAFFIN CELLS".JOURNAL OF BIOLOGICAL CHEMISTRY 267.32(1992):22728-22732.