Structural characterization of the fusion core in syncytin, envelope protein of human endogenous retrovirus family W

doi:10.1016/j.bbrc.2005.04.032

	Structural characterization of the fusion core in syncytin, envelope protein of human endogenous retrovirus family W
	Gong, R ; Peng, XX ; Kang, SL ; Feng, HX ; Huang, JY ; Zhang, WT ; Lin, DH ; Tien, P ; Xiao, GF
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2005-06-17
卷号	331 期号:4 页码:1193-1200
关键词	syncytin fusion human endogenous retrovirus envelope protein heptad repeats trophoblast cell
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2005.04.032
文献子类	Article
英文摘要	Syncytin is a captive retroviral envelope protein, possibly involved in the formation of the placental syncytiotrophoblast layer generated by trophoblast cell fusion at the maternal-fetal interface. We found that syncytin and type I viral envelope proteins shared similar structural profiling, especially in the regions of N- and C-terminal heptad repeats (NHR and CHR). We expressed the predicted regions of NHR (41 aa) and CHR (34 aa) in syncytin as a native single chain (named 2-helix protein) to characterize it. 2-helix protein exists as a trimer and is highly a-helix, thermo-stable, and denatured by low pH. NHR and CHR could form a protease-resistant complex. The complex structure built by the molecular docking demonstrated that NHR and CHR associated in an antiparallel manner. Overall, the 2-helix protein could form a thermo-stable coiled coil trimer. The fusion core structure of syncytin was first demonstrated in endogenous retrovirus. These results support the explanation how syncytin mediates cytotrophoblast cell fusion involved in placental morphogenesis. (c) 2005 Elsevier Inc. All rights reserved.
WOS关键词	CIRCULAR-DICHROISM SPECTRA ; HEPTAD REPEAT REGIONS ; HERV-W ; FUNCTIONAL-CHARACTERIZATION ; INFLUENZA HEMAGGLUTININ ; SECONDARY STRUCTURE ; 6-HELIX BUNDLE ; SPIKE PROTEIN ; CELL-FUSION ; VIRUS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000229234500042
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273852]
专题	中国科学院上海药物研究所
通讯作者	Tien, P
作者单位	1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Meteria Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Gong, R,Peng, XX,Kang, SL,et al. Structural characterization of the fusion core in syncytin, envelope protein of human endogenous retrovirus family W[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,331(4):1193-1200.
APA	Gong, R.,Peng, XX.,Kang, SL.,Feng, HX.,Huang, JY.,...&Xiao, GF.(2005).Structural characterization of the fusion core in syncytin, envelope protein of human endogenous retrovirus family W.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,331(4),1193-1200.
MLA	Gong, R,et al."Structural characterization of the fusion core in syncytin, envelope protein of human endogenous retrovirus family W".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 331.4(2005):1193-1200.