Probucol mediates vascular remodeling after percutaneous transluminal angioplasty via down-regulation of the ERK1/2 signaling pathway

doi:10.1016/j.ejphar.2007.05.050

	Probucol mediates vascular remodeling after percutaneous transluminal angioplasty via down-regulation of the ERK1/2 signaling pathway
	Yang, Yun-Bo ; Yang, Yi-Xin ; Su, Bo ; Tang, Yun-Lian ; Zhu, Bing-Yang ; Hu, Zhuo-Wei ; Li, Gui-Yuan ; Li, Yuan-Jian ; Liao, Duan-Fang
刊名	EUROPEAN JOURNAL OF PHARMACOLOGY
	2007-09-10
卷号	570 期号:1-3 页码:125-134
关键词	Probucol vascular remodeling ERK1/2 VSMC
ISSN号	0014-2999
DOI	10.1016/j.ejphar.2007.05.050
文献子类	Article
英文摘要	Although probucol is known to prevent restenosis by regulating vascular remodeling after percutancous transluminal coronary angioplasty, the mechanisms remain unclear. The present study was designed to investigate whether probucol mediates vascular remodeling via the extracellular signal-regulated kinase 1/2 (ERK1/2) signalling pathway. A rabbit restenosis model was used, in which the New Zealand white rabbits received angioplasty with a 3.5 F angioplasty balloon catheter and the proliferation and migration of smooth muscle cells (SMCs) was induced by oxidized low-density lipoprotein (ox-LDL). We evaluated several vascular remodeling parameters and found that probucol prevented lumen restenosis and mediated expansive remodeling with a remodeling index greater than I and that the proliferation and migration of SMCs was inhibited. Based on Western blot analyses, probucol decreased the expression of phospho-mitogen-activated protein kinase kinases 1 (p-MEK1) and phospho-ERK1/2 and enhanced the expression of mitogen-activated protein (MAP) kinase phosphatase-1 (MKP-1) and caveolin-1. Cells treated with the MEK1 inhibitor PD98059 demonstrated a remarkable suppression of the effects of probucol. Furthermore, immuno fluorescence analysis showed that probucol inhibited the activation of ERK1/2 by preventing its translocation to the nucleus. It was also found that c-myc expression in aortic tissue after angioplasty and the activator protein 1 (AP1) activity in SMCs induced by ox-LDL were decreased with probucol treatment. In conclusion, probucol mediated vascular remodeling to prevent restenosis after angioplasty by down-regulating the ERK1/2 signaling pathway. (c) 2007 Elsevier B.V. All rights reserved.
WOS关键词	SERIAL INTRAVASCULAR ULTRASOUND ; LOW-DENSITY-LIPOPROTEIN ; SMOOTH-MUSCLE-CELLS ; NITRIC-OXIDE ; CORONARY-ARTERY ; BALLOON ANGIOPLASTY ; MAP KINASE ; RESTENOSIS ; EXPRESSION ; ACTIVATION
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000249295100017
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273150]
专题	中国科学院上海药物研究所
通讯作者	Liao, Duan-Fang
作者单位	1.Nanhua Univ, Sch Life Sci & Technol, Inst Pharm & Pharmacol, Div Pharmacoproteom, Hengyang 421001, Peoples R China 2.Cent S Univ, Sch Pharmaceut Sci, Dept Pharmacol, Changsha 410083, Peoples R China 3.Cent S Univ, Canc Res Inst, Changsha 410083, Peoples R China 4.Chinese Acad Sci, Inst Mat Med, Beijing, Peoples R China 5.Peking Union Med Coll, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yun-Bo,Yang, Yi-Xin,Su, Bo,et al. Probucol mediates vascular remodeling after percutaneous transluminal angioplasty via down-regulation of the ERK1/2 signaling pathway[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2007,570(1-3):125-134.
APA	Yang, Yun-Bo.,Yang, Yi-Xin.,Su, Bo.,Tang, Yun-Lian.,Zhu, Bing-Yang.,...&Liao, Duan-Fang.(2007).Probucol mediates vascular remodeling after percutaneous transluminal angioplasty via down-regulation of the ERK1/2 signaling pathway.EUROPEAN JOURNAL OF PHARMACOLOGY,570(1-3),125-134.
MLA	Yang, Yun-Bo,et al."Probucol mediates vascular remodeling after percutaneous transluminal angioplasty via down-regulation of the ERK1/2 signaling pathway".EUROPEAN JOURNAL OF PHARMACOLOGY 570.1-3(2007):125-134.