Neuroprotective Activities of Enzymatically Hydrolyzed Peptides from Porcine Hide Gelatin

	Neuroprotective Activities of Enzymatically Hydrolyzed Peptides from Porcine Hide Gelatin
	Wang, Shaoyun 2; Wang, Deng-Shun 1; Wang, Rui 1,3
刊名	INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
	2008
卷号	1 期号:3 页码:283-293
关键词	Gelatin hydrolysate antioxidative neuroprotective neurotrophic
ISSN号	1940-5901
文献子类	Article
英文摘要	Central nervous system disorders, including cerebrovascular disease, neurodegenerative diseases and head trauma are the most common cause of severe disability in adults and share a number of pathophysiological features. The therapeutic strategy of neuroprotection has been well accepted as one of the promising approaches in treating such brain disorders, and searching for the effective neuroprotective agents is still an open-ended task for neurologists and neuro-pharmacologists. In this study, we report for the first time that the enzymatic hydrolysates from type-B porcine hide gelatin has potent neuroprotective activity against H2O2- or serum deprivation-induced injuries of cultured SH-SY5Y cells. The peptides used in this study were prepared from type-B porcine hide gelatin digested with pepsin and papain. The neuroprotective activity of the porcine hide gelatin hydrolysate (PHH) was evaluated using MTT reduction assay. From the pre-screening of PHH, we found that the whole porcine hide gelatin hydrolysate obtained from papain digestion (PHH-I) showed significant neuroprotective activities (P<0.05). After further separation of PPH-I through SP-Sephadex C-50 and Sephadex G-25, only the fraction with smaller molecular weight from Sephadex G-25 (PHH-Ic) demonstrated potent neuroprotective activities (P<0.01). The active fraction showed a molecular mass between 1,000-3,000Da in SDS-polyacrylamide gel electrophoresis, and was rich in Glycine, Proline and Hydroxyproline in amino acid composition, indicating that peptides with a spectrum of molecular sizes and certain amino acids are critical for the neuroprotective activities of gelatin peptides. The viability of cultured cells treated with gelatin peptides was significantly improved in a dose-dependent manner. Further studies are necessary to establish the neuroprotective activity of hydrolyzed peptides for the neurons in vivo.
资助项目	Education Hall Foundation of Fujian Province, China[JB05040] ; National Natural Science Foundation of China[30572169] ; NIH[AG025722] ; NIH[AG029972] ; Alzheimer Association[IIRG-08-90524]
WOS研究方向	Research & Experimental Medicine
语种	英语
出版者	E-CENTURY PUBLISHING CORP
WOS记录号	WOS:000208700200008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273044]
专题	中国科学院上海药物研究所
通讯作者	Wang, Rui
作者单位	1.Univ Wisconsin, Sch Med & Publ Hlth, Madison, WI 53706 USA 2.Fuzhou Univ, Coll Biosci & Biotechnol, Fuzhou 350002, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Wang, Shaoyun,Wang, Deng-Shun,Wang, Rui. Neuroprotective Activities of Enzymatically Hydrolyzed Peptides from Porcine Hide Gelatin[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2008,1(3):283-293.
APA	Wang, Shaoyun,Wang, Deng-Shun,&Wang, Rui.(2008).Neuroprotective Activities of Enzymatically Hydrolyzed Peptides from Porcine Hide Gelatin.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,1(3),283-293.
MLA	Wang, Shaoyun,et al."Neuroprotective Activities of Enzymatically Hydrolyzed Peptides from Porcine Hide Gelatin".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 1.3(2008):283-293.