Structure and RNA recognition of ribosome assembly factor Utp30

doi:10.1261/rna.062695.117

	Structure and RNA recognition of ribosome assembly factor Utp30
	Hu, Jianfei 1,2,3,4; Zhu, Xing 1; Ye, Keqiong 1,4
刊名	RNA
	2017-12
卷号	23 期号:12 页码:1936-1945
关键词	ribosome assembly 90S preribosome crystal structure RNA-binding protein
ISSN号	1355-8382
DOI	10.1261/rna.062695.117
文献子类	Article
英文摘要	The 90S preribosomes are gigantic early assembly intermediates of small ribosomal subunits. Cryo-EM structures of 90S were recently determined, but many of its components have not been accurately modeled. Here we determine the crystal structure of yeast Utp30, a ribosomal L1 domain-containing protein in 90S, at 2.65 angstrom resolution, revealing a classic two-domain fold. The structure of Utp30 fits well into the cryo-EM density of 90S, confirming its previously assigned location. Utp30 binds to the rearranged helix 41 of 18S rRNA and helix 4 of 5' external transcribed spacer in 90S. Comparison of RNA-binding modes of different L1 domains illustrates that they consistently recognize a short RNA duplex with the concaved surface of domain I, but are versatile in RNA recognition outside the core interface. Cic1 is a paralog of Utp30 associating with large subunit preribosomes. Utp30 and Cic1 share similar RNA-binding modes, suggesting that their distinct functions may be executed by a single protein in other organisms. Deletion of Utp30 does not affect the composition of 90S. The nonessential role of Utp30 could be ascribed to its peripheral localization and redundant interactions in 90S.
资助项目	National Natural Science Foundation of China[91540201] ; National Natural Science Foundation of China[31430024] ; National Natural Science Foundation of China[31325007] ; Chinese Academy of Sciences[XDB08010203] ; National Key R&D Program of China[2017YFA0504600] ; CAS[00000000]
WOS关键词	EUKARYOTIC RIBOSOME ; SACCHAROMYCES-CEREVISIAE ; NUCLEAR EXPORT ; BIOGENESIS ; YEAST ; SUBUNITS
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
WOS记录号	WOS:000415372800017
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272389]
专题	中国科学院上海药物研究所
通讯作者	Ye, Keqiong
作者单位	1.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Key Lab RNA Biol, Beijing 100101, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Jianfei,Zhu, Xing,Ye, Keqiong. Structure and RNA recognition of ribosome assembly factor Utp30[J]. RNA,2017,23(12):1936-1945.
APA	Hu, Jianfei,Zhu, Xing,&Ye, Keqiong.(2017).Structure and RNA recognition of ribosome assembly factor Utp30.RNA,23(12),1936-1945.
MLA	Hu, Jianfei,et al."Structure and RNA recognition of ribosome assembly factor Utp30".RNA 23.12(2017):1936-1945.