Metabolic changes at the early stage of sepsis induced by cecal ligation and puncture in rats and the interventional effects of Huang-Lian-Jie-Du-Tang

doi:10.1016/j.jchromb.2015.08.009

	Metabolic changes at the early stage of sepsis induced by cecal ligation and puncture in rats and the interventional effects of Huang-Lian-Jie-Du-Tang
	Wei, Yufen 1,2; Gao, Na 3; Zhang, Zhongxiao 2; Zu, Xianpeng 2; Hu, Zhenlin 2; Zhang, Weidong 2; Yin, Jun 3; Liu, Xinru 2
刊名	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
	2016-07-15
卷号	1026 页码:176-182
ISSN号	1873-376X
DOI	10.1016/j.jchromb.2015.08.009
文献子类	Article
英文摘要	Sepsis is a disease with high mortality that requires rapid diagnosis and treatment. This study used a metabolomic approach to profile the metabolic changes at the early stage of sepsis induced by cecal ligation and puncture (CLP) in rats and investigated the interventional effects of Huang-Lian-Jie-Du-Tang (HLJDT). Male SD rats were intragastrically administered 270mg/kg HLJDT 2h prior to CLP, serum extracts were profiled by liquid chromatography/quadrupole time-of-flight mass spectrometer (LC-Q-TOF-MS) and multivariate analytical (MVA) methods were employed to evaluate the metabolic changes of extracts. A Partial Least-Squares Discriminant Analysis (PLS-DA) score plot indicated that septic rats undergo significant metabolic changes 2h after CLP, and HLJDT administration could reverse the metabolic changes induced by CLP. Sixteen biomarkers involved in amino acid metabolism, unsaturated fatty acid metabolism, purine metabolism, and lipid metabolism were identified after Orthogonal Partial Least-Squares Analysis (OPLS). Among the 16 metabolites, 10 were regulated by HLJDT. This study established the foundation for further research of the early diagnosis biomarkers and therapeutic evaluation biomarkers discovery of sepsis.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266551]
专题	中国科学院上海药物研究所
通讯作者	Yin, Jun; Liu, Xinru
作者单位	1.National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China; 2.School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China; 3.School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China
推荐引用方式 GB/T 7714	Wei, Yufen,Gao, Na,Zhang, Zhongxiao,et al. Metabolic changes at the early stage of sepsis induced by cecal ligation and puncture in rats and the interventional effects of Huang-Lian-Jie-Du-Tang[J]. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,2016,1026:176-182.
APA	Wei, Yufen.,Gao, Na.,Zhang, Zhongxiao.,Zu, Xianpeng.,Hu, Zhenlin.,...&Liu, Xinru.(2016).Metabolic changes at the early stage of sepsis induced by cecal ligation and puncture in rats and the interventional effects of Huang-Lian-Jie-Du-Tang.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,1026,176-182.
MLA	Wei, Yufen,et al."Metabolic changes at the early stage of sepsis induced by cecal ligation and puncture in rats and the interventional effects of Huang-Lian-Jie-Du-Tang".Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 1026(2016):176-182.