SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza

doi:10.1038/srep22852

	SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza
	Zhou, Yangyun 1; Sun, Wei 2; Chen, Junfeng 1; Tan, Hexin 3; Xiao, Ying 1; Li, Qing 1; Ji, Qian 3; Gao, Shouhong 1; Chen, Li 1; Chen, Shilin 2
刊名	Scientific reports
	2016-03-07
卷号	6 页码:22852
ISSN号	2045-2322
DOI	10.1038/srep22852
文献子类	Article
英文摘要	Salvia miltiorrhiza Bunge, which contains tanshinones and phenolic acids as major classes of bioactive components, is one of the most widely used herbs in traditional Chinese medicine. Production of tanshinones and phenolic acids is enhanced by methyl jasmonate (MeJA). Transcription factor MYC2 is the switch of jasmontes signaling in plants. Here, we focused on two novel JA-inducible genes in S. miltiorrhiza, designated as SmMYC2a and SmMYC2b, which were localized in the nucleus. SmMYC2a and SmMYC2b were also discovered to interact with SmJAZ1 and SmJAZ2, implying that the two MYC2s might function as direct targets of JAZ proteins. Ectopic RNA interference (RNAi)-mediated knockdown experiments suggested that SmMYC2a/b affected multiple genes in tanshinone and phenolic acid biosynthetic pathway. Besides, the accumulation of tanshinones and phenolic acids was impaired by the loss of function in SmMYC2a/b. Meanwhile, SmMYC2a could bind with an E-box motif within SmHCT6 and SmCYP98A14 promoters, while SmMYC2b bound with an E-box motif within SmCYP98A14 promoter, through which the regulation of phenolic acid biosynthetic pathway might achieve. Together, these results suggest that SmMYC2a and SmMYC2b are JAZ-interacting transcription factors that positively regulate the biosynthesis of tanshinones and Sal B with similar but irreplaceable effects.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266464]
专题	中国科学院上海药物研究所
作者单位	1.Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; 2.Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, China; 3.Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
推荐引用方式 GB/T 7714	Zhou, Yangyun,Sun, Wei,Chen, Junfeng,et al. SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza[J]. Scientific reports,2016,6:22852.
APA	Zhou, Yangyun.,Sun, Wei.,Chen, Junfeng.,Tan, Hexin.,Xiao, Ying.,...&Chen, Wansheng.(2016).SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza.Scientific reports,6,22852.
MLA	Zhou, Yangyun,et al."SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza".Scientific reports 6(2016):22852.