Metabolic profiles and pharmacokinetics of picroside I in rats by liquid chromatography combined with electrospray ionization tandem mass spectrometry | |
Xiong, Kai2,3; Ju, Zhengcai1; Zhang, Tong2,4; Wang, Zhengtao1; Han, Han2,4 | |
刊名 | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
2018-09-15 | |
卷号 | 1095页码:157-165 |
ISSN号 | 1873-376X |
DOI | 10.1016/j.jchromb.2018.07.034 |
文献子类 | Article |
英文摘要 | Picroside I is an iridoid glycoside derived from Picrorhiza kurroa Royle ex Benth and Picrorhiza scrophulariiflora Pennell and characterized by many biological activities. In this study, a fast, selective, and sensitive UHPLC-MS/MS method was developed and validated to determine picroside I in rat plasma. Analytes were separated by using an ACQUITY UPLC® BEH C18 (2.1 × 50 mm, 1.7 μm) column at a running time of 2 min. Selected reaction monitoring (SRM) transitions were m/z 491.1 → 147.1 for picroside I and m/z 511.1 → 235.1 for the internal standard in a negative ion mode. The established UHPLC-MS/MS method achieved good linearity for picroside I within the range of 0.1-500 ng/mL. The validated method was successfully applied for the pharmacokinetic analysis of picroside I in rats after oral administration. Fifteen metabolites of picroside I were tentatively identified through ultra-high-performance chromatography/tandem quadrupole time-of-flight mass spectrometry, and four metabolites were identified by comparing with the standards. Besides, nine of these metabolites were discovered for the first time. The proposed metabolic pathways of picroside I in vivo can be divided into four parts, namely, phase I reaction of picroside I, including hydroxylation and deoxygenation; phase II reaction of picroside I, including glucuronidation, sulfation, and methylation; phase I biotransformations of metabolites, such as reduction and hydroxylation; and phase II biotransformations of metabolites, such as glucuronidation and sulfation. These results could offer insights into the effectiveness and toxicity of picroside I. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/266455] |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Zhang, Tong; Han, Han |
作者单位 | 1.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201210, China; 2.Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China; 3.School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China; 4.School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China |
推荐引用方式 GB/T 7714 | Xiong, Kai,Ju, Zhengcai,Zhang, Tong,et al. Metabolic profiles and pharmacokinetics of picroside I in rats by liquid chromatography combined with electrospray ionization tandem mass spectrometry[J]. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,2018,1095:157-165. |
APA | Xiong, Kai,Ju, Zhengcai,Zhang, Tong,Wang, Zhengtao,&Han, Han.(2018).Metabolic profiles and pharmacokinetics of picroside I in rats by liquid chromatography combined with electrospray ionization tandem mass spectrometry.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,1095,157-165. |
MLA | Xiong, Kai,et al."Metabolic profiles and pharmacokinetics of picroside I in rats by liquid chromatography combined with electrospray ionization tandem mass spectrometry".Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 1095(2018):157-165. |
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