Radix Paeoniae Alba increases serum estrogen level and up-regulates estrogen receptor expression in uterus and vagina of immature/ovariectomized mice

doi:10.1002/ptr.6205

	Radix Paeoniae Alba increases serum estrogen level and up-regulates estrogen receptor expression in uterus and vagina of immature/ovariectomized mice
	Xu, Ying 1; Li, Xin 1; Chen, Ting 1; Qu, Ya-Kun 1; Zheng, Hong-Xia 1; Zhang, Zi-Jia 2; Zhao, Yuan 1; Lin, Na 1
刊名	Phytotherapy research : PTR
	2018-10-29
ISSN号	1099-1573
DOI	10.1002/ptr.6205
文献子类	Article
英文摘要	Radix Paeoniae Alba (RPA) is widely used in clinical treatment for gynecological diseases, particularly abnormal menstruation, menstrual pain, and breast tenderness; however, no scientific evidence base links RPA to estrogen replacement therapy. In this study, we characterize estrogenic activity of RPA using immature and ovariectomized (OVX) mice together with in vitro studies focus on estrogen receptor (ER) pathway for molecular mechanism. RPA treatments demonstrated significant estrogenic activity, as indicated by promoting the development of uterus and vagina in immature mice, reversing the atrophy of uterus and vagina in OVX mice, up-regulating the expressions of ERα and ERβ at protein and mRNA level in reproductive tissues. Meanwhile, RPA significantly increased serum estradiol and clearly decreased serum luteinizing hormone and follicle-stimulating hormone of immature/OVX mice. Moreover, RPA could induce ER positive MCF-7 cell from S-phase to G2 stage and induce proliferation and no influence on ER negative MDA-MB-231 cell. RPA could bind with ERα and ERβ and significantly stimulate ERα/β-estrogen response element (ERE) luciferase reporter gene expression. All activities were inhibited by the ER antagonist ICI 182,780. This study illustrates RPA exerts estrogenic effects by stimulating biosynthesis of estrogen in circulation, up-regulating ERs in target tissues, and mimicking the estrogen through ER-ERE-dependent pathway.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266436]
专题	中国科学院上海药物研究所
作者单位	1.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China; 2.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China
推荐引用方式 GB/T 7714	Xu, Ying,Li, Xin,Chen, Ting,et al. Radix Paeoniae Alba increases serum estrogen level and up-regulates estrogen receptor expression in uterus and vagina of immature/ovariectomized mice[J]. Phytotherapy research : PTR,2018.
APA	Xu, Ying.,Li, Xin.,Chen, Ting.,Qu, Ya-Kun.,Zheng, Hong-Xia.,...&Lin, Na.(2018).Radix Paeoniae Alba increases serum estrogen level and up-regulates estrogen receptor expression in uterus and vagina of immature/ovariectomized mice.Phytotherapy research : PTR.
MLA	Xu, Ying,et al."Radix Paeoniae Alba increases serum estrogen level and up-regulates estrogen receptor expression in uterus and vagina of immature/ovariectomized mice".Phytotherapy research : PTR (2018).