The effect of phytic acid on tight junctions in the human intestinal Caco-2 cell line and its mechanism

doi:10.1016/j.ejps.2015.09.009

	The effect of phytic acid on tight junctions in the human intestinal Caco-2 cell line and its mechanism
	Fu, Qingxue 1,2; Wang, Huizhen 2; Xia, Mengxin 2; Deng, Bing 2; Shen, Hongyi 2; Ji, Guang 3; Li, Guowen 4; Xie, Yan 2,3
刊名	European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
	2015-12-01
卷号	80 页码:1-8
ISSN号	1879-0720
DOI	10.1016/j.ejps.2015.09.009
文献子类	Article
英文摘要	This study investigated the effect of phytic acid (IP6), a potential absorption enhancer of flavonoid components, on tight junction (TJ) integrity in Caco-2 cell monolayers and its possible mechanisms. Transepithelial electrical resistance (TEER) across the monolayers decreased rapidly, and the flux of fluorescein sodium (a paracellular marker) increased after treating with IP6 in a concentration-dependent manner. Confocal microscopy results showed that IP6 produced a concentration-dependent attenuation in the distribution of occludin, ZO-1, and claudin-1. Immunoblot analysis revealed that IP6 could down-regulate the expression level of these TJ proteins, which resulted in the opening of TJ. Additionally, the divalent cations Ca(2+) and Mg(2+) influenced the IP6-induced distribution of occludin, ZO-1, and claudin-1 in different directions, which enhanced barrier function. In conclusion, IP6 can decrease the integrity of Caco-2 cell monolayers by modulating the TJ proteins' localization and down-regulating the expression levels of TJ proteins including claudin-1, occludin, and ZO-1; the reduction effects of divalent cations such as Ca(2+) and Mg(2+) on the regulation of TJ induced by IP6 should be addressed. The present work will offer some useful guidance for the application of IP6 in drug delivery area.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266428]
专题	中国科学院上海药物研究所
通讯作者	Li, Guowen; Xie, Yan
作者单位	1.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2.Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 3.Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China; 4.Pharmacy Department, Shanghai TCM-integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200082, China
推荐引用方式 GB/T 7714	Fu, Qingxue,Wang, Huizhen,Xia, Mengxin,et al. The effect of phytic acid on tight junctions in the human intestinal Caco-2 cell line and its mechanism[J]. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences,2015,80:1-8.
APA	Fu, Qingxue.,Wang, Huizhen.,Xia, Mengxin.,Deng, Bing.,Shen, Hongyi.,...&Xie, Yan.(2015).The effect of phytic acid on tight junctions in the human intestinal Caco-2 cell line and its mechanism.European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences,80,1-8.
MLA	Fu, Qingxue,et al."The effect of phytic acid on tight junctions in the human intestinal Caco-2 cell line and its mechanism".European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 80(2015):1-8.