The effects of jatrorrhizine on contractile responses of rat ileum

doi:10.1016/j.ejphar.2011.05.002

	The effects of jatrorrhizine on contractile responses of rat ileum
	Yuan, JianYe; Zhou, JiYan; Hu, ZhiBi; Ji, Guang; Xie, JianQun; Wu, DaZheng
刊名	European journal of pharmacology
	2011-08-01
卷号	663 期号:1-3 页码:74-9
ISSN号	1879-0712
DOI	10.1016/j.ejphar.2011.05.002
文献子类	Article
英文摘要	This study was designed to evaluate the effect of jatrorrhizine on smooth muscle contractions isolated from rat ileum longitudinal muscles. Jatrorrhizine increased the amplitude of spontaneous contractions of ileum longitudinal muscles in concentration-dependent manner with an EC(50) of 30.0±8.4μM. Preincubation of ileum strips with atropine (1μM), 4-diphenyllacetoxy-N (2-chloriethyl)-piperidine (4-DAMP, 1μM) or darifenacin (1μM) significantly inhibited acetylcholine (0.1μM)- and jatrorrhizine (100μM)-induced ileum longitudinal muscle contractions, whereas they were not affected by AF-DX116 (1μM) or hexamethonium (100μM). Pretreatment with SB204070 (1μM) rather than 3-tropanyl-indole-3-carboxyleat (tropisetron, 1μM) significantly inhibited 5-HT (10μM)-induced ileum longitudinal muscle contractions. In contrast, jatrorrhizine-induced ileum longitudinal muscle contractions were not inhibited by tropisetron or SB204070. Furthermore, jatrorrhizine-induced ileum longitudinal muscle contractions were strongly inhibited by nifedipine (1μM), and also attenuated by removal of extracellular Ca(2+), U73122 (1μM), ruthenium red (50μM) or 2-aminoethoxydiphenylborate (2-APB, 10μM). Taken together, jatrorrhizine-elicited spontaneous contractions in rat ileum longitudinal muscles are mediated by activation of acetylcholine receptors, mostly the M(3) receptor. Ca(2+) influx through L-type Ca(2+) channel is significantly contributed to jatrorrhizine-elicited spontaneous contractions, and Ca(2+) release via IP(3) and ryanodine pathways are also involved.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266386]
专题	中国科学院上海药物研究所
作者单位	Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
推荐引用方式 GB/T 7714	Yuan, JianYe,Zhou, JiYan,Hu, ZhiBi,et al. The effects of jatrorrhizine on contractile responses of rat ileum[J]. European journal of pharmacology,2011,663(1-3):74-9.
APA	Yuan, JianYe,Zhou, JiYan,Hu, ZhiBi,Ji, Guang,Xie, JianQun,&Wu, DaZheng.(2011).The effects of jatrorrhizine on contractile responses of rat ileum.European journal of pharmacology,663(1-3),74-9.
MLA	Yuan, JianYe,et al."The effects of jatrorrhizine on contractile responses of rat ileum".European journal of pharmacology 663.1-3(2011):74-9.