A Highly Sensitive Non-Radioactive Activity Assay for AMP-Activated Protein Kinase (AMPK)

doi:10.3390/mps1010003

	A Highly Sensitive Non-Radioactive Activity Assay for AMP-Activated Protein Kinase (AMPK)
	Yan, Yan 1,2; Gu, Xin 1,2; Xu, H Eric 1,2; Melcher, Karsten 1,2
刊名	Methods and protocols
	2018-03
卷号	1 期号:1
ISSN号	2409-9279
DOI	10.3390/mps1010003
文献子类	Article
英文摘要	While many methods exist to quantitatively determine protein kinase activities, P-based radioactive assays remain the workhorse of many laboratories due to their high sensitivity, high signal to noise ratio, lack of interference by fluorescent and light-absorbing small molecules, and easy quantitation. Here, we demonstrate that the interaction between the yeast Rad53 Forkhead-associated (FHA) domain and a peptide optimized for phosphorylation by AMP-Activated Protein Kinase (AMPK), which has previously been exploited for the generation of intracellular phosphorylation sensors, can serve as a readout for a highly sensitive two-step AMPK AlphaScreen kinase assay with exceptional signal-to-noise ratio.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266304]
专题	中国科学院上海药物研究所
通讯作者	Yan, Yan; Gu, Xin; Xu, H Eric; Melcher, Karsten
作者单位	1.eric 2.Laboratory of Structural Sciences and Laboratory of Structural Biology and Biochemistry, Center of Cancer and Cell Biology, Van Andel Research Institute, 333 Bostwick Avenue Northeast, Grand Rapids, MI 49503, USA;
推荐引用方式 GB/T 7714	Yan, Yan,Gu, Xin,Xu, H Eric,et al. A Highly Sensitive Non-Radioactive Activity Assay for AMP-Activated Protein Kinase (AMPK)[J]. Methods and protocols,2018,1(1).
APA	Yan, Yan,Gu, Xin,Xu, H Eric,&Melcher, Karsten.(2018).A Highly Sensitive Non-Radioactive Activity Assay for AMP-Activated Protein Kinase (AMPK).Methods and protocols,1(1).
MLA	Yan, Yan,et al."A Highly Sensitive Non-Radioactive Activity Assay for AMP-Activated Protein Kinase (AMPK)".Methods and protocols 1.1(2018).