Antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae under methylmercury exposure

doi:10.1016/j.cbpc.2019.108589

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae under methylmercury exposure
	Ren, Zhonghua 2,4; Liu, Jinhu 3,4; Huang, Wei 1; Cao, Liang 3,4; Cui, Wenting 2,4; Dou, Shuozeng 2,3,4
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
	2019-11-01
卷号	225 页码:11
关键词	Metal accumulation Larval growth Biochemical markers Gene transcription Oxidative stress Immunotoxicity
ISSN号	1532-0456
DOI	10.1016/j.cbpc.2019.108589
通讯作者	Cao, Liang(caoliang@qdio.ac.cn) ; Dou, Shuozeng(szdou@qdio.ac.cn)
英文摘要	Methylmercury (MeHg) is a highly toxic contaminant in coastal environments and poses threats to marine fish in early life stages (ELSs). However, MeHg toxicity to fish embryos and larvae is not well investigated. This study investigated the antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae exposed to waterborne MeHg (0, 0.1, 1.0 and 10.0 mu g L-1) for 35 days, from embryogenesis to settlement. The results revealed that metal accumulation in the larvae was positively correlated with MeHg concentration, reduced larval growth and survival. The activities of catalase and glutathione reductase were significantly increased at 10.0 mu g L-1, while glutathione peroxidase activity and lipid peroxidation level were significantly increased at concentrations over 1.0 mu g L-1. The corresponding antioxidant-related genes were upregulated under MeHg exposure (cat and gpx at 10.0 mu g L-1; gr over 1.0 mu g L-1). Lysozyme content was significantly increased, but immunoglobulin M content was significantly decreased at 10.0 mu g L-1. The immune-related genes were significantly upregulated (hsp70 at 0.1 and 10.0 mu g L-1; lzm and il-1 beta over 1.0 mu g L-1; tnf-alpha and il-6 at 10.0 mu g L-1) or downregulated (igm, over 0.1 mu g L-1). Overall, MeHg exposure induced oxidative stress and caused immunotoxicity at concentrations over 1.0 mu g L-1 and 10.0 mu g L-1, respectively. The transcription of selected genes correlated with the corresponding biochemical markers in response to MeHg toxicity. These findings improve our knowledge to better understand the mechanisms by which marine fish at ELSs cope with oxidative stress and immunotoxicity induced by MeHg.
资助项目	National Key Basic Research Program[2015CB453302] ; National Natural Science Foundation of China[41406176] ; National Natural Science Foundation of China[41306112]
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000488304600018
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/163220]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Cao, Liang; Dou, Shuozeng
作者单位	1.State Ocean Adm, Inst Oceanog 2, Key Lab Marine Ecosyst & Biogeochem, Hangzhou 310000, Zhejiang, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Ren, Zhonghua,Liu, Jinhu,Huang, Wei,et al. Antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae under methylmercury exposure[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2019,225:11.
APA	Ren, Zhonghua,Liu, Jinhu,Huang, Wei,Cao, Liang,Cui, Wenting,&Dou, Shuozeng.(2019).Antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae under methylmercury exposure.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,225,11.
MLA	Ren, Zhonghua,et al."Antioxidant defenses and immune responses of flounder Paralichthys olivaceus larvae under methylmercury exposure".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 225(2019):11.