Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis

doi:10.3389/fpls.2018.01092

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis
	Yu, Bin 1,4; Niu, Jianfeng 2,3,4; Feng, Jianhua 4; Xu, Meiling 4; Xie, Xiujun 2,3,4; Gu, Wenhui 2,3,4; Gao, Shan 2,3,4; Wang, Guangce 2,3,4
刊名	FRONTIERS IN PLANT SCIENCE
	2018-07-25
卷号	9 页码:13
关键词	electron transportation ferredoxin-NADP(+) reductase Pyropia yezoensis stress responding environmental acclimation
ISSN号	1664-462X
DOI	10.3389/fpls.2018.01092
通讯作者	Niu, Jianfeng(jf_niu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要	Pyropia yezoensis can survive the severe water loss that occurs during low tide, making it an ideal species to investigate the acclimation mechanism of intertidal seaweed to special extreme environments. In this study, we determined the effects of high salinity on photosynthesis using increasing salinity around algal tissues. Both electron transport rates, ETR (I) and ETR (II), showed continuous decreases as the salinity increased. However, the difference between these factors remained relatively stable, similar to the control. Inhibitor experiments illustrated that there were at least three different cyclic electron transport pathways. Under conditions of severe salinity, NAD(P) H could be exploited as an endogenous electron donor to reduce the plastoquinone pool in Py. yezoensis. Based on these findings, we next examined how these different cyclic electron transport (CETs) pathways were coordinated by cloning the gene (HM370553) for ferredoxin-NADP C oxidoreductase (FNR). A phylogenetic tree was constructed, and the evolutionary relationships among different FNRs were evaluated. The results indicated that the Py. yezoensis FNR showed a closer relationship with cyanobacterial FNR. The results of both real-time polymerase chain reaction and western blotting showed that the enzyme was upregulated under 90-120 h salinity. Due to the structurefunction correlations in organism, Py. yezoensis FNR was proposed to be involved in NAD(P) H-dependent Fd C reduction under severe salinity conditions. Thus, through the connection between different donors bridged by FNR, electrons were channeled toward distinct routes according to the different metabolic demands. This was expected to make the electron transfer in the chloroplasts become more flexible and to contribute greatly to acclimation of Py. yezoensis to the extreme variable environments in the intertidal zone.
资助项目	National Natural Science Foundation of China[41476140] ; National Natural Science Foundation of China[41776150] ; China Agriculture Research System-50 and National Key Research and Development Project[2016YFC1400600] ; Science and Technology Plan of Jiangsu Province[BE2016330] ; Strategic Leading Science and Technology Projects of Chinese Academy of Sciences[XDA11020705]
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000439717600001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/163501]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Niu, Jianfeng; Wang, Guangce
作者单位	1.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Yu, Bin,Niu, Jianfeng,Feng, Jianhua,et al. Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis[J]. FRONTIERS IN PLANT SCIENCE,2018,9:13.
APA	Yu, Bin.,Niu, Jianfeng.,Feng, Jianhua.,Xu, Meiling.,Xie, Xiujun.,...&Wang, Guangce.(2018).Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis.FRONTIERS IN PLANT SCIENCE,9,13.
MLA	Yu, Bin,et al."Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis".FRONTIERS IN PLANT SCIENCE 9(2018):13.