Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum

doi:10.1186/s12934-019-1214-x

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum
	Wu, Songcui3,4,5 ; Gu, Wenhui 3,4,5; Huang, Aiyou 3,4,5; Li, Yuanxiang 3,4,5; Kumar, Manoj 2; Lim, Phaik Eem 1; Huan, Li3,4,5 ; Gao, Shan 3,4,5; Wang, Guangce 3,4,5
刊名	MICROBIAL CELL FACTORIES
	2019-09-23
卷号	18 期号:1 页码:16
关键词	Glucose-6-phosphate dehydrogenase Overexpression Antisense knockdown CO2 Phaeodactylum tricornutum Lipid accumulation Algal growth rate
DOI	10.1186/s12934-019-1214-x
通讯作者	Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要	Background Numerous studies have shown that stress induction and genetic engineering can effectively increase lipid accumulation, but lead to a decrease of growth in the majority of microalgae. We previously found that elevated CO2 concentration increased lipid productivity as well as growth in Phaeodactylum tricornutum, along with an enhancement of the oxidative pentose phosphate pathway (OPPP) activity. The purpose of this work directed toward the verification of the critical role of glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme in the OPPP, in lipid accumulation in P. tricornutum and its simultaneous rapid growth rate under high-CO2 (0.15%) cultivation. Results In this study, G6PDH was identified as a target for algal strain improvement, wherein G6PDH gene was successfully overexpressed and antisense knockdown in P. tricornutum, and systematic comparisons of the photosynthesis performance, algal growth, lipid content, fatty acid profiles, NADPH production, G6PDH activity and transcriptional abundance were performed. The results showed that, due to the enhanced G6PDH activity, transcriptional abundance and NAPDH production, overexpression of G6PDH accompanied by high-CO2 cultivation resulted in a much higher of both lipid content and growth in P. tricornutum, while knockdown of G6PDH greatly decreased algal growth as well as lipid accumulation. In addition, the total proportions of saturated and unsaturated fatty acid, especially the polyunsaturated fatty acid eicosapentaenoic acid (EPA; C20:5, n-3), were highly increased in high-CO2 cultivated G6PDH overexpressed strains. Conclusions The successful of overexpression and antisense knockdown of G6PDH well demonstrated the positive influence of G6PDH on algal growth and lipid accumulation in P. tricornutum. The improvement of algal growth, lipid content as well as polyunsaturated fatty acids in high-CO2 cultivated G6PDH overexpressed P. tricornutum suggested this G6PDH overexpression-high CO2 cultivation pattern provides an efficient and economical route for algal strain improvement to develop algal-based biodiesel production.
资助项目	National Natural Science Foundation of China[41806171] ; National Natural Science Foundation of China[41876158] ; National Key R&D Program of China[2018YFD0901501-1] ; Haikou Marine Economic Demonstration City Industrial Chain Collaborative Innovation Project[HHCL201811] ; Tianjin Marine Economic Demonstration[BHSF2017-21]
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	BMC
WOS记录号	WOS:000487442400002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/162956]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Guangce
作者单位	1.Univ Malaya, IOES, Kuala Lumpur 50603, Malaysia 2.UTS, Fac Sci, Climate Change Cluster, Sydney, NSW, Australia 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wu, Songcui,Gu, Wenhui,Huang, Aiyou,et al. Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum[J]. MICROBIAL CELL FACTORIES,2019,18(1):16.
APA	Wu, Songcui.,Gu, Wenhui.,Huang, Aiyou.,Li, Yuanxiang.,Kumar, Manoj.,...&Wang, Guangce.(2019).Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum.MICROBIAL CELL FACTORIES,18(1),16.
MLA	Wu, Songcui,et al."Elevated CO2 improves both lipid accumulation and growth rate in the glucose-6-phosphate dehydrogenase engineered Phaeodactylum tricornutum".MICROBIAL CELL FACTORIES 18.1(2019):16.