Programming chain-growth copolymerization of DNA hairpin tiles for in-vitro hierarchical supramolecular organization

doi:10.1038/s41467-019-09004-4

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Programming chain-growth copolymerization of DNA hairpin tiles for in-vitro hierarchical supramolecular organization
	Zhang, HL ; Wang, Y ; Zhang, H ; Liu, XG ; Lee, A ; Huang, QL ; Wang, F ; Chao, J ; Liu, HJ ; Li, J
刊名	NATURE COMMUNICATIONS
	2019
卷号	10 期号:-页码:—
关键词	TARGETED TRANSPORT ACCURATE DESIGN NANOTECHNOLOGY NANOTUBES POLYMERIZATION NANOPARTICLES HYDROGELS DELIVERY TUBE
ISSN号	2041-1723
DOI	10.1038/s41467-019-09004-4
文献子类	期刊论文
英文摘要	Formation of biological filaments via intracellular supramolecular polymerization of proteins or protein/nucleic acid complexes is under programmable and spatiotemporal control to maintain cellular and genomic integrity. Here we devise a bioinspired, catassembly-like isothermal chain-growth approach to copolymerize DNA hairpin tiles (DHTs) into nanofilaments with desirable composition, chain length and function. By designing metastable DNA hairpins with shape-defining intramolecular hydrogen bonds, we generate two types of DHT monomers for copolymerization with high cooperativity and low dispersity indexes. Quantitative single-molecule dissection methods reveal that catalytic opening of a DHT motif harbouring a toehold triggers successive branch migration, which autonomously propagates to form copolymers with alternate tile units. We find that these shape-defined supramolecular nanostructures become substrates for efficient endocytosis by living mammalian cells in a stiffness-dependent manner. Hence, this catassembly-like in-vitro reconstruction approach provides clues for understanding structure-function relationship of biological filaments under physiological and pathological conditions.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31776]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China; 2.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200240, Peoples R China; 3.Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China; 4.Univ Calif Berkeley, Inst Quantitat Biosci QB3, Kavli Energy Nanosci Inst Berkeley, Howard Hughes Med Inst,Dept Chem,Dept Phys,Dept M, Berkeley, CA 94720 USA; 5.Chinese Acad Sci, Bioimaging Ctr, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China; 6.Chinese Acad Sci, Div Phys Biol, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China; 7.Shanghai Jiao Tong Univ, Joint Res Ctr Precis Med, Fengxian Hosp, Affiliated Sixth Peoples Hosp South Campus, Shanghai 201499, Peoples R China; 8.Nanjing Univ Posts & Telecommun, Jiangsu Natl Synergist Innovat Ctr Adv Mat SICAM, Key Lab Organ Elect & Informat Displays, Nanjing 210023, Jiangsu, Peoples R China; 9.Nanjing Univ Posts & Telecommun, Jiangsu Natl Synergist Innovat Ctr Adv Mat SICAM, Inst Adv Mat, Nanjing 210023, Jiangsu, Peoples R China; 10.East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Green Chem & Chem Proc, Shanghai 200241, Peoples R China
推荐引用方式 GB/T 7714	Zhang, HL,Wang, Y,Zhang, H,et al. Programming chain-growth copolymerization of DNA hairpin tiles for in-vitro hierarchical supramolecular organization[J]. NATURE COMMUNICATIONS,2019,10(-):—.
APA	Zhang, HL.,Wang, Y.,Zhang, H.,Liu, XG.,Lee, A.,...&Fan, CH.(2019).Programming chain-growth copolymerization of DNA hairpin tiles for in-vitro hierarchical supramolecular organization.NATURE COMMUNICATIONS,10(-),—.
MLA	Zhang, HL,et al."Programming chain-growth copolymerization of DNA hairpin tiles for in-vitro hierarchical supramolecular organization".NATURE COMMUNICATIONS 10.-(2019):—.