Investigation of inner ear anatomy in mouse using X-ray phase contrast tomography

doi:10.1002/jemt.23121

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Investigation of inner ear anatomy in mouse using X-ray phase contrast tomography
	Yin, HX ; Zhang, P ; Wang, Z ; Liu, YF ; Liu, Y ; Xiao, TQ ; Yang, ZH ; Xian, JF ; Zhao, PF ; Li, J
刊名	MICROSCOPY RESEARCH AND TECHNIQUE
	2019
卷号	82 期号:7 页码:953—960
关键词	3-DIMENSIONAL RECONSTRUCTION VISUALIZATION OTOCONIA COCHLEA VIVO MICROSCOPY EVOLUTION MODELS MIDDLE MICE
ISSN号	1059-910X
DOI	10.1002/jemt.23121
文献子类	期刊论文
英文摘要	A thorough understanding of inner ear anatomy is important for investigators. However, investigation of the mouse inner ear is difficult due to the limitations of imaging techniques. X-ray phase contrast tomography increases contrast 100-1,000 times compared with conventional X-ray imaging. This study aimed to investigate inner ear anatomy in a fresh post-mortem mouse using X-ray phase contrast tomography and to provide a comprehensive atlas of microstructures with less tissue deformation. All experiments were performed in accordance with our institution's guidelines on the care and use of laboratory animals. A fresh mouse cadaver was scanned immediately after sacrifice using an inline phase contrast tomography system. Slice images were reconstructed using a filtered back-projection (FBP) algorithm. Standardized axial and coronal planes were adjusted with a multi-planar reconstruction method. Some three-dimensional (3D) objects were reconstructed by surface rendering. The characteristic features of microstructures, including otoconia masses of the saccular and utricular maculae, superior and inferior macula cribrosae, single canal, modiolus, and osseous spiral lamina, were described in detail. Spatial positions and relationships of the vestibular structures were exhibited in 3D views. This study investigated mouse inner ear anatomy and provided a standardized presentation of microstructures. In particular, otoconia masses were visualized in their natural status without contrast for the first time. The comprehensive anatomy atlas presented in this study provides an excellent reference for morphology studies of the inner ear.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31681]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Capital Med Univ, Beijing Friendship Hosp, Dept Radiol, Beijing, Peoples R China; 2.Capital Med Univ, Beijing Tongren Hosp, Dept Radiol, Beijing, Peoples R China; 3.Chinese Acad Med Sci, Peking Union Med Coll, Comparat Med Ctr, Beijing, Peoples R China; 4.Chinese Acad Med Sci, Inst Lab Anim Sci, Beijing, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Yin, HX,Zhang, P,Wang, Z,et al. Investigation of inner ear anatomy in mouse using X-ray phase contrast tomography[J]. MICROSCOPY RESEARCH AND TECHNIQUE,2019,82(7):953—960.
APA	Yin, HX.,Zhang, P.,Wang, Z.,Liu, YF.,Liu, Y.,...&Wang, ZC.(2019).Investigation of inner ear anatomy in mouse using X-ray phase contrast tomography.MICROSCOPY RESEARCH AND TECHNIQUE,82(7),953—960.
MLA	Yin, HX,et al."Investigation of inner ear anatomy in mouse using X-ray phase contrast tomography".MICROSCOPY RESEARCH AND TECHNIQUE 82.7(2019):953—960.