In-Situ Configuration Studies on Segmented DNA Origami Nanotubes

doi:10.1002/cbic.201800727

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	In-Situ Configuration Studies on Segmented DNA Origami Nanotubes
	Zhu, BW ; Guo, JY ; Zhang, LX ; Pan, MC ; Jing, XX ; Wang, LH ; Liu, XG ; Zuo, XL
刊名	CHEMBIOCHEM
	2019
卷号	20 期号:12 页码:1508—1513
关键词	SINGLE NANOSTRUCTURES FILAMENTS FLEXIBILITY TRANSPORT CHANNELS DEVICES DESIGN SHAPE
ISSN号	1439-4227
DOI	10.1002/cbic.201800727
文献子类	期刊论文
英文摘要	One-dimensional nanotubes are of considerable interest in materials and biochemical sciences. A particular desire is to create DNA nanotubes with user-defined structural features and biological relevance, which will facilitate the application of these nanotubes in the controlled release of drugs, templating of other materials into linear arrays and the construction of artificial membrane channels. However, little is known about the structures of assembled DNA nanotubes in solution. Here we report an in situ exploration of segmented DNA nanotubes, composed of multiple units with set length distributions, by using synchrotron small-angle X-ray scattering (SAXS). Through joint experimental and theoretical studies, we show that the SAXS data are highly informative in the context of heterogeneous mixtures of DNA nanotubes. The structural parameters obtained by SAXS are in good agreement with those determined by atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering (DLS). In particular, the SAXS data revealed important structural information on these DNA nanotubes, such as the in-solution diameters (approximate to 25 nm), which could be obtained only with difficulty by use of other methods. Our results establish SAXS as a reliable structural analysis method for long DNA nanotubes and could assist in the rational design of these structures.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31675]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Appl Phys,CAS Key Lab Interfacial P, Bioimaging Ctr,Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China; 2.Jiading Dist Cent Hosp, Shanghai 201800, Peoples R China; 3.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200240, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China; 5.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China;
推荐引用方式 GB/T 7714	Zhu, BW,Guo, JY,Zhang, LX,et al. In-Situ Configuration Studies on Segmented DNA Origami Nanotubes[J]. CHEMBIOCHEM,2019,20(12):1508—1513.
APA	Zhu, BW.,Guo, JY.,Zhang, LX.,Pan, MC.,Jing, XX.,...&Zuo, XL.(2019).In-Situ Configuration Studies on Segmented DNA Origami Nanotubes.CHEMBIOCHEM,20(12),1508—1513.
MLA	Zhu, BW,et al."In-Situ Configuration Studies on Segmented DNA Origami Nanotubes".CHEMBIOCHEM 20.12(2019):1508—1513.