Cancer-Specific MicroRNA Analysis with a Nonenzymatic Nucleic Acid Circuit

doi:10.1021/acsami.9b01653

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Cancer-Specific MicroRNA Analysis with a Nonenzymatic Nucleic Acid Circuit
	Zhu, D ; Lu, B ; Zhu, Y ; Ma, ZH ; Wei, YQ ; Su, S ; Wang, LH ; Song, SP ; Zhu, Y ; Wang, LH
刊名	ACS APPLIED MATERIALS & INTERFACES
	2019
卷号	11 期号:12 页码:11220—11226
关键词	ENZYME-FREE SIGNAL AMPLIFICATION DNA NANOSTRUCTURES GASTRIC-CANCER ISOTHERMAL AMPLIFICATION GOLD NANOPARTICLES POLYMORPHISMS CONSTRUCTION BIOSENSOR RNA
ISSN号	1944-8244
DOI	10.1021/acsami.9b01653
文献子类	期刊论文
英文摘要	Sensitive detection of gastric cancer-related biomarkers in human serum provides a promising means for early cancer diagnosis. Herein, we report the design of a nucleic acid circuit for gastric cancer-related microRNA-27a (miRNA-27a) detection based on dual toehold-mediated circular strand displacement amplification (CSDA). In the presence of miRNA-27a, the hybridization between miRNA-27a and probe DNA on magnetic beads through toehold 1 leads to the release of fluorescent DNA and the exposure of a new toehold 2 on linker DNA. After hybridization with catalytic DNA, CSDA is initiated and target miRNA-27a is released to participate in the next cyclic reaction; therefore, a greatly enhanced fluorescence signal is produced. The efficient magnetic separation makes the sensitive detection of miRNA-27a be accomplished within 45 min. With the efficient CSDA, the detection limit of the system (0.8 pM) is similar to 100 folds lower than that of the system based on strand displacement without CSDA (79.3 pM). Furthermore, the system also showed good stability and sensitivity to discriminate single-base mismatch, which allows the detection of miRNA-27a in human serum samples. This study provides a novel platform and approach for the rapid quantitative determination of miRNA, which has great potential in clinical diagnosis and disease treatment.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31497]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Chinese Acad Sci, Div Phys Biol, Shanghai 201800, Peoples R China; 2.Nanjing Univ Posts & Telecommun, Jiangsu Key Lab Biosensors, IAM, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 210023, Jiangsu, Peoples R China; 3.Nanjing Univ Posts & Telecommun, Key Lab Organ Elect & Informat Displays, IAM, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 210023, Jiangsu, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Bioimaging Ctr,Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China
推荐引用方式 GB/T 7714	Zhu, D,Lu, B,Zhu, Y,et al. Cancer-Specific MicroRNA Analysis with a Nonenzymatic Nucleic Acid Circuit[J]. ACS APPLIED MATERIALS & INTERFACES,2019,11(12):11220—11226.
APA	Zhu, D.,Lu, B.,Zhu, Y.,Ma, ZH.,Wei, YQ.,...&Chao, J.(2019).Cancer-Specific MicroRNA Analysis with a Nonenzymatic Nucleic Acid Circuit.ACS APPLIED MATERIALS & INTERFACES,11(12),11220—11226.
MLA	Zhu, D,et al."Cancer-Specific MicroRNA Analysis with a Nonenzymatic Nucleic Acid Circuit".ACS APPLIED MATERIALS & INTERFACES 11.12(2019):11220—11226.