Effects of nanobubbles on peptide self-assembly

doi:10.1039/c8nr06142d

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Effects of nanobubbles on peptide self-assembly
	Wang, YJ ; Shen, ZW ; Guo, Z ; Hu, J ; Zhang, Y
刊名	NANOSCALE
	2018
卷号	10 期号:42 页码:20007-20012
关键词	AIR-WATER-INTERFACE NEURODEGENERATIVE DISEASES PROTEIN AGGREGATION AMYLOID FIBRILS BETA(2)-MICROGLOBULIN MECHANISM SURFACES BIOLOGY FIBERS GROWTH
ISSN号	2040-3364
DOI	10.1039/c8nr06142d
文献子类	期刊论文
英文摘要	It is believed that the aggregation of amyloid proteins or peptides is promoted by the presence of an air-water interface, and substantial evidence suggests that the characteristics of the air-water interface play critical roles in foam-induced protein aggregation during foam fractionation. However, the effects of the air-water interface on the self-assembly of amyloid-like peptides have not yet been elucidated clearly at the nanometer scale. In this work, air nanobubbles produced in water solution were employed for studying interfacial effects on the self-assembly of a model amyloid peptide termed P11. An atomic force microscopy study showed that the air nanobubbles induced the formation of peptide fibrils with a 9-13 nm helix structure in the P11 solution. Thioflavin T fluorescence and circular dichroism spectroscopic analysis indicated that the nanobubbles induced the change of the peptide conformation to a beta-sheet structure. Based on these observations, we have proposed a mechanism to explain how the nanobubbles affect the self-assembly of the P11 peptide at the nanometer scale. Since air nanobubbles are present in water solutions in addition to an air-water interface in normal experiments in vitro, our results indicate that nanobubbles must be taken into account to achieve a complete understanding of protein aggregation events.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/30920]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China
推荐引用方式 GB/T 7714	Wang, YJ,Shen, ZW,Guo, Z,et al. Effects of nanobubbles on peptide self-assembly[J]. NANOSCALE,2018,10(42):20007-20012.
APA	Wang, YJ,Shen, ZW,Guo, Z,Hu, J,&Zhang, Y.(2018).Effects of nanobubbles on peptide self-assembly.NANOSCALE,10(42),20007-20012.
MLA	Wang, YJ,et al."Effects of nanobubbles on peptide self-assembly".NANOSCALE 10.42(2018):20007-20012.