Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging

doi:10.1039/c9tc01929d

	Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging
	Zhou, Hui 1,3,6; Yang, Han 6; Tang, Lin 6; Wang, Yue 6; Li, Yang 6; Liu, Nan 4; Zeng, Xiaodong 3,6; Yan, Yu 4; Wu, Junzhu 4; Chen, Shizhen 5
刊名	JOURNAL OF MATERIALS CHEMISTRY C
	2019-08-21
卷号	7 期号:31 页码:9448-9454
ISSN号	2050-7526
DOI	10.1039/c9tc01929d
英文摘要	The integration of molecular imaging probes into a biodegradable, noncytotoxic and biocompatible nanoplatform is very promising for high efficacy tumor diagnosis but is still a great challenge. Herein, lactoferrin (Lf), an iron-binding glycoprotein, was applied to develop a novel molecular platform. Biocompatible Mn-loaded apolactoferrin Mn2+-Apo-Lf-PEG was first prepared. The first dual-modality imaging NIR-II/MRI nanoprobe (H-dot) was then constructed by linking a NIR-II fluorescent probe (CH1055) to the surface of Mn2+-Apo-Lf-PEG via glutaraldehyde cross linking. Cellular studies showed that the obtained H-dot exhibited low toxicity, excellent stability, biocompatibility, significantly enhanced T1-weighted magnetic resonance imaging (MRI) and superior NIR-II imaging optical properties. The H-dot was subsequently evaluated in subcutaneous HepG2 liver xenografts and subcutaneous/orthotopic U87MG glioblastoma xenografts, revealing its excellent dual imaging properties in small-animal models for the first time, such as high tumor specificity and contrast, good tumor uptake, and accurate tumor delineation. These particular properties of the Mn-loaded apolactoferrin dotmake it a promisingmolecular platform for the development of novel imaging probes and clinical translation.
资助项目	National Natural Science Foundation of China[81773674] ; National Natural Science Foundation of China[81573383] ; National Natural Science Foundation of China[21763002] ; NSFHP[2017CFA024] ; NSFHP[2017CFB711] ; NSFHP[2016ACA126] ; Health Commission of Hubei Province Scientific Research Project[WJ2019M177] ; Health Commission of Hubei Province Scientific Research Project[WJ2019M178] ; Tibet Autonomous Region Science and Technology Plan Project Key Project[XZ201901-GB-11] ; Shenzhen Science and Technology Research Grant[JCYJ20170303170809222] ; Fundamental Research Funds for the Central Universities
WOS关键词	CONTRAST AGENT ; NANOPARTICLES ; FLUORESCENCE ; MANGANESE ; FLUOROPHORES ; WAVELENGTHS ; LACTOFERRIN ; COMPLEXES ; CHROMIUM ; COBALT
WOS研究方向	Materials Science ; Physics
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000480277200041
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; NSFHP ; NSFHP ; Health Commission of Hubei Province Scientific Research Project ; Health Commission of Hubei Province Scientific Research Project ; Tibet Autonomous Region Science and Technology Plan Project Key Project ; Tibet Autonomous Region Science and Technology Plan Project Key Project ; Shenzhen Science and Technology Research Grant ; Shenzhen Science and Technology Research Grant ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; NSFHP ; NSFHP ; Health Commission of Hubei Province Scientific Research Project ; Health Commission of Hubei Province Scientific Research Project ; Tibet Autonomous Region Science and Technology Plan Project Key Project ; Tibet Autonomous Region Science and Technology Plan Project Key Project ; Shenzhen Science and Technology Research Grant ; Shenzhen Science and Technology Research Grant ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; NSFHP ; NSFHP ; Health Commission of Hubei Province Scientific Research Project ; Health Commission of Hubei Province Scientific Research Project ; Tibet Autonomous Region Science and Technology Plan Project Key Project ; Tibet Autonomous Region Science and Technology Plan Project Key Project ; Shenzhen Science and Technology Research Grant ; Shenzhen Science and Technology Research Grant ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; NSFHP ; NSFHP ; Health Commission of Hubei Province Scientific Research Project ; Health Commission of Hubei Province Scientific Research Project ; Tibet Autonomous Region Science and Technology Plan Project Key Project ; Tibet Autonomous Region Science and Technology Plan Project Key Project ; Shenzhen Science and Technology Research Grant ; Shenzhen Science and Technology Research Grant ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/14806]
专题	中国科学院武汉物理与数学研究所
通讯作者	Hong, Xuechuan; Xiao, Yuling
作者单位	1.Tibet Univ, Coll Med, Innovat Ctr Tradit Tibetan Med Modernizat & Qual, Lhasa 850000, Peoples R China 2.Univ Aberdeen, Dept Chem, Aberdeen, Scotland 3.Wuhan Univ, Shenzhen Inst, Shenzhen 518057, Peoples R China 4.Wuhan Univ, Ctr Expt Basic Med Educ, Hubei Prov Key Lab Dev Originated Dis, Wuhan 430071, Hubei, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance Wuhan, Wuhan 430071, Hubei, Peoples R China 6.Wuhan Univ, Key Lab Combinatorial Biosynth & Drug Discovery, Hubei Prov Engn & Technol Res Ctr Fluorinated Pha, MOE,Sch Pharmaceut Sci, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Hui,Yang, Han,Tang, Lin,et al. Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging[J]. JOURNAL OF MATERIALS CHEMISTRY C,2019,7(31):9448-9454.
APA	Zhou, Hui.,Yang, Han.,Tang, Lin.,Wang, Yue.,Li, Yang.,...&Xiao, Yuling.(2019).Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging.JOURNAL OF MATERIALS CHEMISTRY C,7(31),9448-9454.
MLA	Zhou, Hui,et al."Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging".JOURNAL OF MATERIALS CHEMISTRY C 7.31(2019):9448-9454.