Solution NMR structure of CGL2373, a polyketide cyclase-like protein from Corynebacterium glutamicum

doi:10.1002/prot.25771

	Solution NMR structure of CGL2373, a polyketide cyclase-like protein from Corynebacterium glutamicum
	Cai, Cong 2,3; Nie, Yao 3,4; Gong, Yixuan 3,4; Li, Shuangli 3,4; Ramelot, Theresa A.1; Kennedy, Michael A.1; Yue, Xiali 2; Zhu, Jiang 3; Liu, Maili 3; Yang, Yunhuang 3
刊名	PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
	2019-07-17
页码	5
关键词	aromatic ring Bet v1 Polyketide_cyc2 PYR1-like START-like
ISSN号	0887-3585
DOI	10.1002/prot.25771
英文摘要	Protein CGL2373 from Corynebacterium glutamicum was previously proposed to be a member of the polyketide_cyc2 family, based on amino-acid sequence and secondary structure features derived from NMR chemical shift assignments. We report here the solution NMR structure of CGL2373, which contains three alpha-helices and one antiparallel beta-sheet and adopts a helix-grip fold. This structure shows moderate similarities to the representative polyketide cyclases, TcmN, WhiE, and ZhuI. Nevertheless, unlike the structures of these homologs, CGL2373 structure looks like a half-open shell with a much larger pocket, and key residues in the representative polyketide cyclases for binding substrate and catalyzing aromatic ring formation are replaced with different residues in CGL2373. Also, the gene cluster where the CGL2373-encoding gene is located in C. glutamicum contains additional genes encoding nucleoside diphosphate kinase, folylpolyglutamate synthase, and valine-tRNA ligase, different from the typical gene cluster encoding polyketide cyclase in Streptomyces. Thus, although CGL2373 is structurally a polyketide cyclase-like protein, the function of CGL2373 may differ from the known polyketide cyclases and needs to be further investigated. The solution structure of CGL2373 lays a foundation for in silico ligand screening and binding site identifying in future functional study.
资助项目	National Institute of General Medical Sciences[U54-GM094597] ; National Natural Science Foundation of China[21575155] ; National Natural Science Foundation of China[21703283]
WOS关键词	CRYSTAL-STRUCTURE ; AROMATIC POLYKETIDES ; MOLECULAR-BASIS ; BIOSYNTHESIS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	WILEY
WOS记录号	WOS:000476016200001
资助机构	National Institute of General Medical Sciences ; National Institute of General Medical Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institute of General Medical Sciences ; National Institute of General Medical Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institute of General Medical Sciences ; National Institute of General Medical Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Institute of General Medical Sciences ; National Institute of General Medical Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/14734]
专题	中国科学院武汉物理与数学研究所
通讯作者	Yue, Xiali; Zhu, Jiang
作者单位	1.Miami Univ, Northeast Struct Genom Consortium, Dept Chem & Biochem, Oxford, OH 45056 USA 2.Huazhong Agr Univ, Coll Sci, Dept Chem, Wuhan 430070, Hubei, Peoples R China 3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom Mol Phys, Key Lab Magnet Resonance Biol Syst, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cai, Cong,Nie, Yao,Gong, Yixuan,et al. Solution NMR structure of CGL2373, a polyketide cyclase-like protein from Corynebacterium glutamicum[J]. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,2019:5.
APA	Cai, Cong.,Nie, Yao.,Gong, Yixuan.,Li, Shuangli.,Ramelot, Theresa A..,...&Yang, Yunhuang.(2019).Solution NMR structure of CGL2373, a polyketide cyclase-like protein from Corynebacterium glutamicum.PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,5.
MLA	Cai, Cong,et al."Solution NMR structure of CGL2373, a polyketide cyclase-like protein from Corynebacterium glutamicum".PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2019):5.