Protein stability analysis in ionic liquids by F-19 NMR

doi:10.1007/s00216-019-01804-3

	Protein stability analysis in ionic liquids by F-19 NMR
	Cheng, Kai; Wu, Qiong; Jiang, Ling; Liu, Maili; Li, Conggang
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2019-07-01
卷号	411 期号:19 页码:4929-4935
关键词	Ionic liquids Protein stability quantification F-19 NMR
ISSN号	1618-2642
DOI	10.1007/s00216-019-01804-3
英文摘要	Ionic liquids have been extensively used as environmentally friendly solvents for enzymatic reactions and other biological systems. Understanding the mechanism of how ionic liquids affect protein stability is crucial for the biological reaction processes and protein storage using ionic liquids as solvents. Although effects of ionic liquids on protein stability have been studied, equilibrium thermodynamics of protein stability in ionic liquids has not been quantitatively measured. Herein, we utilized F-19 NMR to measure the equilibrium thermodynamics of protein stability in ionic liquid [C-4-mim]Br. Our results show that proteins are significantly destabilized in [C-4-mim]Br ionic liquids. Our results suggest that F-19 NMR provides a simple and effective way to study the thermodynamics of protein stability in ionic liquids. F-19 NMR will be applicable to facilitate the protein-protein interaction study in ionic liquids.
资助项目	Ministry of Science and Technology of China[2017YFA0505400] ; Innovation team of Hubei Province grant[2016CFA002] ; National Natural Sciences Foundation of China[21575156] ; K.C. Wong Education Foundation[QYZDJ-SSW-SLH027] ; Chinese Academy of Sciences[QYZDJ-SSW-SLH027]
WOS关键词	ANTARCTICA LIPASE-B ; THERMAL-STABILITY ; HOFMEISTER SERIES ; CYTOCHROME-C ; STABILIZATION ; EXTRACTION ; INTERMEDIATE ; DISSOLUTION ; IMPROVEMENT ; HEMOGLOBIN
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000474354500058
资助机构	Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Innovation team of Hubei Province grant ; Innovation team of Hubei Province grant ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Innovation team of Hubei Province grant ; Innovation team of Hubei Province grant ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Innovation team of Hubei Province grant ; Innovation team of Hubei Province grant ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Innovation team of Hubei Province grant ; Innovation team of Hubei Province grant ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K.C. Wong Education Foundation ; K.C. Wong Education Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/14636]
专题	中国科学院武汉物理与数学研究所
通讯作者	Li, Conggang
作者单位	Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math,Natl Ctr Magnet Resonance, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Kai,Wu, Qiong,Jiang, Ling,et al. Protein stability analysis in ionic liquids by F-19 NMR[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(19):4929-4935.
APA	Cheng, Kai,Wu, Qiong,Jiang, Ling,Liu, Maili,&Li, Conggang.(2019).Protein stability analysis in ionic liquids by F-19 NMR.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(19),4929-4935.
MLA	Cheng, Kai,et al."Protein stability analysis in ionic liquids by F-19 NMR".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.19(2019):4929-4935.