Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb

doi:10.1016/j.ijbiomac.2019.04.087

	Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb
	Zhi, Fei 1,2,3; Yang, Tian-Le 2; Wang, Qiang 1; Jiang, Bin 4; Wang, Zhong-Ping 1,3; Zhang, Jing 1,3; Chen, Yun-Zhong 1,3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2019-07-15
卷号	133 页码:1201-1209
关键词	Dioscorea opposita Thunb Polysaccharide Isolation Monosaccharide composition Structural characterization Antioxidant activity
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2019.04.087
英文摘要	A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China. DOW-B was found to be neutral in charge, with an average molecular weight of 5623 Da. Monosaccharide composition analysis by GC-MS revealed that DOTP-B was a hetero-polysaccharide consisting of glucose and galactose at a molar ratio of 14.6:1.0. Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra. Highly correlated results demonstrated that the main chain of DOTP-B consisted of -> 4)-alpha-D-glc(1 -> residues, with about 6% internal -> 6)-beta-D-gal(1 -> residues. The antioxidant activity of DOTP-B was also evaluated as the EC50 values against DPPH and PTIO radicals were 2.1 +/- 0.1 mg/mL and 1.6 +/- 0.1 mg/mL, respectively. Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries. (C) 2019 Published by Elsevier B.V.
资助项目	Research Fund of the Major Projects of Technology Innovation of Department of Science and Technology, Hubei Province[2016ACA145]
WOS关键词	ANTIOXIDANT ACTIVITY ; HEPATOPROTECTIVE ACTIVITIES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000472685700131
资助机构	Research Fund of the Major Projects of Technology Innovation of Department of Science and Technology, Hubei Province ; Research Fund of the Major Projects of Technology Innovation of Department of Science and Technology, Hubei Province ; Research Fund of the Major Projects of Technology Innovation of Department of Science and Technology, Hubei Province ; Research Fund of the Major Projects of Technology Innovation of Department of Science and Technology, Hubei Province ; Research Fund of the Major Projects of Technology Innovation of Department of Science and Technology, Hubei Province ; Research Fund of the Major Projects of Technology Innovation of Department of Science and Technology, Hubei Province ; Research Fund of the Major Projects of Technology Innovation of Department of Science and Technology, Hubei Province ; Research Fund of the Major Projects of Technology Innovation of Department of Science and Technology, Hubei Province
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/14560]
专题	中国科学院武汉物理与数学研究所
通讯作者	Chen, Yun-Zhong
作者单位	1.Hubei Univ Chinese Med, Coll Pharm, Wuhan 430065, Hubei, Peoples R China 2.Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China 3.Hubei Tradit Chinese Med & Hlth Food Engn Res Ctr, Wuhan 430065, Hubei, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Zhi, Fei,Yang, Tian-Le,Wang, Qiang,et al. Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,133:1201-1209.
APA	Zhi, Fei.,Yang, Tian-Le.,Wang, Qiang.,Jiang, Bin.,Wang, Zhong-Ping.,...&Chen, Yun-Zhong.(2019).Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,133,1201-1209.
MLA	Zhi, Fei,et al."Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 133(2019):1201-1209.