Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage

doi:10.1038/s41421-019-0101-2

	Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage
	Guan, Zeyuan 3,4; Pei, Kai 3,4; Wang, Jing 3,4; Cui, Yongqing 3,4; Zhu, Xiang 3,4; Su, Xiang 3,4; Zhou, Yuanbao 3,4; Zhang, Delin 3,4; Tang, Chun 1,5; Yin, Ping 3,4
刊名	CELL DISCOVERY
	2019-05-28
卷号	5 页码:14
ISSN号	2056-5968
DOI	10.1038/s41421-019-0101-2
英文摘要	A newly identified arbitrium communication system regulates the lysis-to-lysogeny decision in a Bacillus bacteriophage. This system contains an arbitrium hexapeptide as a signal, the cellular receptor AimR, and the lysogenic negative regulator AimX. AimR specifically targets the downstream DNA to activate aimX gene expression. The arbitrium peptide binds to AimR, inhibiting its DNA-binding to promote phage lysogeny. Recently, we and other groups have elucidated how arbitrium peptide sensed by AimR. However, the molecular mechanisms of DNA recognition by AimR and the regulation of its DNA-binding activity by the peptide remain largely unknown. Here, we report the crystal structure of the AimR-DNA complex at 2.1 angstrom resolution. The N-terminal H T H motif recognizes the palindromic DNA sequence, buttressed by interactions between positively charged residues and the DNA phosphate groups. The DNA-bound AimR assembles a more closed dimer than the peptide-bound form. Single-molecule FRET and crosslinking assays revealed that the AimR protein samples both open and closed conformations in solution. Arbitrium peptide binding induces a closed-to-open conformational change of AimR, eliminating DNA targeting. Our structural and functional analysis provides new insights into the DNA recognition mechanism of AimR and its regulation by the arbitrium peptide in the context of phage lysis-lysogeny decisions.
资助项目	National Key R&D Program of China[2018YFA0507700] ; National Natural Science Foundation of China[81601/42] ; Fundamental Research Funds for the Central Universities[2662014BQ028] ; Fundamental Research Funds for the Central Universities[2662017PY031]
WOS关键词	MACROMOLECULAR CRYSTALLOGRAPHY BEAMLINE ; TO-CELL COMMUNICATION ; QUORUM ; MODEL ; PLCR
WOS研究方向	Cell Biology
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000471023100001
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/14532]
专题	中国科学院武汉物理与数学研究所
通讯作者	Zou, Tingting
作者单位	1.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China 2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Hubei, Peoples R China 3.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China 4.Huazhong Agr Univ, Natl Ctr Plant Gene Res, Wuhan 430070, Hubei, Peoples R China 5.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Guan, Zeyuan,Pei, Kai,Wang, Jing,et al. Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage[J]. CELL DISCOVERY,2019,5:14.
APA	Guan, Zeyuan.,Pei, Kai.,Wang, Jing.,Cui, Yongqing.,Zhu, Xiang.,...&Zou, Tingting.(2019).Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage.CELL DISCOVERY,5,14.
MLA	Guan, Zeyuan,et al."Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage".CELL DISCOVERY 5(2019):14.